We narrowed to 12,459 results for: 109

1. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ALK6 K231R

   Plasmid

   #80884

   Purpose

   mammalian expression of ALK6 K231R

   Depositor

   Aristidis Moustakas

   Article

   Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K231R (kinase-deficient)

   Promoter

   CMV

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-puro /BRAF-V600E

   Plasmid

   #131724

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Cyclin A2

   Plasmid

   #215048

   Purpose

   Expresses EGFP-Cyclin A2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNA2 (CCNA2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-POLE1

   Plasmid

   #215140

   Purpose

   Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   mCherry_FWR

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acid 80-86 and 211-217 …

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PL-CRISPR.EFS.tRFP-gIfngr1-1

   Plasmid

   #166479

   Purpose

   Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   gRNA for mouse Ifngr1 (Ifngr1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL-CRISPR.EFS.tRFP-gIfngr1-2

   Plasmid

   #166480

   Purpose

   Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   gRNA for mouse Ifngr1 (Ifngr1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

   Plasmid

   #196196

   Purpose

   homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
   

   Use

   Homology repair donor

   Tags

   V5-APEX2-mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-SKP2

   Plasmid

   #215092

   Purpose

   Expresses FLAG-SKP2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCUabaa-Flag-PLYS1

    Plasmid

    #236786

    Purpose

    MCU-MCUB fusion in abaa pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MCUabab-Flag-pLYS1

    Plasmid

    #236788

    Purpose

    MCU-MCUB fusion in abab pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

    Plasmid

    #216250

    Purpose

    HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human SEC61B (SEC61B Synthetic, Human, HDR template)
    

    Use

    CRISPR; Hdr template

    Tags

    BSD.P2A.miniIAA7.3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRY2high-mCherry-Raf1

    Plasmid

    #104064

    Purpose

    Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2high-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2PHR E490R

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-APEX2

    Plasmid

    #215127

    Purpose

    Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APEX2 (APEX2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-SLC27A4_STOP

    Plasmid

    #161449

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC27A4 (SLC27A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. EGFP-Cyclin B1

    Plasmid

    #215049

    Purpose

    Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNB1 (CCNB1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Cyclin E1

    Plasmid

    #215053

    Purpose

    Expresses EGFP-Cyclin E1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-puro/RAF1

    Plasmid

    #131725

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG_SARS-CoV2 ORF8 C-V5-tag

    Plasmid

    #179990

    Purpose

    Mammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 ORF8 (ORF8 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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