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Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

Plasmid

#105196

Purpose

targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

Depositor

Peter Mombaerts

Article

Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

Insert

Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)

Use

Mouse Targeting

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-Drosha(W1258)

Plasmid

#171535

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

a Drosha stopping at W1258

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Drosha (P426)

Plasmid

#171537

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

a Drosha mutant starting from P426

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Drosha (E419)

Plasmid

#171534

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

a Drosha mutant stopping at E419

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-Drosha-FLAG(delta6)

Plasmid

#171532

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Article

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

small deletions of Drosha_1271A to 1276G_

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 Na

Plasmid

#124928

Purpose

For expression of a.a. 1-68 of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1-W300A

Plasmid

#123301

Purpose

Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

W300A

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/T35A-IRES-Puro

Plasmid

#120571

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, T35A

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

Plasmid

#120573

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, Y40A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K/E37A-IRES-Puro

Plasmid

#120572

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K, E37A

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFL His-I3 V41A / W43A

Plasmid

#113515

Purpose

Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Tags

6*His

Expression

Insect

Mutation

V41A /W43A

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB1-1/1

Plasmid

#91563

Purpose

Protein expression and purification of human SH3 domain construct CACNB1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB1-1/1 (CACNB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159E

Plasmid

#105311

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S666A

Plasmid

#105308

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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hASXL(1-115)-luciferase

Plasmid

#106427

Purpose

Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

addition sex combs like 1 ASXL1 (ASXL1 Human)

Tags

luciferase

Expression

Mammalian

Mutation

amino acids 1-115 fused to luciferase

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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