Addgene

Showing: 4941 - 4960 of 28326 results

Results per page:

Sort results by:

px335 Slc1a3 nickase 2

Plasmid

#129411

Purpose

Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

Depositor

Walker Jackson

Article

Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

Insert

sgRNA targeting Slc1a3 (Slc1a3 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-RG-Linker-eGFP

Plasmid

#176071

Purpose

EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

RG

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-KR-Linker-eGFP

Plasmid

#176066

Purpose

EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

KR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4 3xFLAG Halo G-Linker nsp1

Plasmid

#175426

Purpose

Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

G-linker nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

G-linker in place of the RNase destabilization do…

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4 3xFLAG Halo R119A K120A nsp1

Plasmid

#175428

Purpose

Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 R119A K120A

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants R119A K120A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

Plasmid

#175427

Purpose

Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E55A E57A K58A

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E55A E57A K58A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA4 3xFLAG Halo E36A E37A nsp1

Plasmid

#175429

Purpose

Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E36A E37A

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E36A E37A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmpr2ΔEC gRNA resistant

Plasmid

#163413

Purpose

Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The extracellular domain (27-150 aa) is deleted. …

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmpr2ΔTail gRNA resistant

Plasmid

#163412

Purpose

Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The C-terminal tail domain (530-1038 aa) is delet…

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQlinkG2-PTPIP51(236-470)

Plasmid

#170534

Purpose

Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Use

Tags

GST tag and His tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQlinkH-PTPIP51(236-470)

Plasmid

#170530

Purpose

Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YF

Plasmid

#171459

Purpose

Expresses YF, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP-FKBP12

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mYF

Plasmid

#171460

Purpose

Expresses mYF, a CiCE of NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP(A207K)-FKBP12

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mActZ-FmCh

Plasmid

#171465

Purpose

Expresses mActZ-FmCh, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Use

Tags

6xHis and V5

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PWPXLD-EGFR-P667A

Plasmid

#133750

Purpose

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Mutant P667A EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

proline 667 converted to alanine (Please see depo…

Promoter

EF1-alpha

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-EGxxFP-CD55

Plasmid

#153959

Purpose

Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)

Use

A plasmid specific for egxxfp assay

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CAG promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge