We narrowed to 11,030 results for: Kars;

1. pLentiN MmuPV1 E6

   Plasmid

   #87661

   Purpose

   Lentiviral expression of MmuPV1 E6

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   MmuPV1 E6
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCMV MmuPV1 E6

   Plasmid

   #87653

   Purpose

   Flag/HA tagged MmuPV1 E6

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   MmuPV1 E6
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YCp33-YDL183c-HA

   Plasmid

   #70164

   Purpose

   yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   YDL183c (217 nucleotides upstream of the ATG)

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pSG5-p85α-ΔiSH2-C

   Plasmid

   #39499

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   p85 ΔiSH2-C (PIK3R1 Bovine)
   

   Use

   Eukaryotic

   Tags

   Expression

   Mutation

   N488I, ΔiSH2-C = deletion of AA494-582

   Promoter

   Available Since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pGEX2TK E7 del EDE

   Plasmid

   #13675

   Depositor

   Karl Munger

   Article

   Jones et al Genes Dev. 1997 Aug 15. 11(16):2101-11.

   Insert

   HPV 16 E7 del EDE (E7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   del aa 35-37

   Promoter

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. GFP-LacI

   Plasmid

   #183920

   Purpose

   Lac repressor labeled with GFP; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   GFP-LacI (lacI Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   SV2

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRE3G-Bi-β-chain

   Plasmid

   #133731

   Purpose

   Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   b-Chain_gePSI
   ZsGreen1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (startcodon + a…

   Promoter

   pTRE3G-Bi

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-hNox4

   Plasmid

   #69352

   Purpose

   expresses human Nox4 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

   Insert

   hNOX4 (NOX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-HA-pcDNA3.1-

    Plasmid

    #52535

    Purpose

    enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Zeo-mEGFP

    Plasmid

    #214145

    Purpose

    Expresses the monomeric EGFP in eukaryotic cells

    Depositor

    Bertrand Collet

    Article

    Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A207K L221K F223R

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDMS151A_Tet-On-3G

    Plasmid

    #191577

    Purpose

    ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Tet-On(R) 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-mNox4

    Plasmid

    #69353

    Purpose

    expresses mouse Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    mNox4 (Nox4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid

    #20938

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAsp-AMA-gpdA-ptr

    Plasmid

    #191016

    Purpose

    CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Type

    Empty backbone

    Use

    Unspecified; Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX.TRE3G.AdProt

    Plasmid

    #131687

    Purpose

    A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3GV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Promoter

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RGS-6xHis-pcDNA3.1-

    Plasmid

    #52534

    Purpose

    enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Type

    Empty backbone

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMDIAI

    Plasmid

    #51655

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    apramycin antibiotic resistance gene
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-CAG-hChR2-H134R-tdTomato

    Plasmid

    #28017

    Purpose

    AAV-mediated expression of hChR2(H134R) for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Has Service

    AAV Retrograde

    Insert

    humanized ChR2-H134R-tdTomato
    

    Use

    AAV; Adeno associate viral

    Tags

    tdTomato

    Expression

    Mutation

    H134R

    Promoter

    CAG

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KU70-eGFP

    Plasmid

    #213517

    Purpose

    Expresses human KU70-eGFP in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    human KU70-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 GFP-h-PKD2

    Plasmid

    #83451

    Purpose

    GFP-tagged PC2 for Eukaryotic expression (883)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-2 (PKD2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKEK3395

    Plasmid

    #234079

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PERK 1: PERK.WT.9E10.pCDNA

    Plasmid

    #21814

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEXPqcxip-hSURF4-FLAG

    Plasmid

    #159139

    Purpose

    Hu SURF4 -flag mammalian expression Gateway vector.

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    surfeit 4 (SURF4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (cytomegalovirus) enhancer and the MSV (mouse…

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tdTomato-LacI

    Plasmid

    #183921

    Purpose

    Lac repressor labeled with tdTomato; can be used to label lacO sites

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdTomato-LacI (lacI Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-p50(1-435)

    Plasmid

    #44747

    Depositor

    Gourisankar Ghosh

    Article

    Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

    Insert

    p50 (Nfkb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    AA 1-435

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPRIME-dsRed-shscramble

    Plasmid

    #71384

    Purpose

    Expresses dsRed and scramble shRNA under the control of the CMV promoter in eukaryotic cells

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    CMV promoter-dsRed-mir30-shscramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-hSyn-eNpHR 3.0-EYFP

    Plasmid

    #26775

    Purpose

    3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Insert

    eNpHR 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Trafficking Signal (TS) ER Export Signal

    Promoter

    hSyn

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKEK3238

    Plasmid

    #234092

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfCherry2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E117Q/T127I/G219A

    Promoter

    pJ23100-BsaI

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKEK3228

    Plasmid

    #234090

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    aeBlue
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-CaMKIIa-C1C2-EYFP

    Plasmid

    #35519

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-mNoxo1

    Plasmid

    #58338

    Purpose

    expresses mouse Noxo1 in mammalian cells

    Depositor

    Botond Banfi, Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    NADPH oxidase organizer 1 (Noxo1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NLS-tdPCP-GFP

    Plasmid

    #183934

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-LacI-NLS-VP16

    Plasmid

    #103836

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-LacI-NLS-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Flag-HA-EIF3S5

    Plasmid

    #22546

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    EIF3S5 (EIF3F Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge