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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term 3C eGFP StrepII

Plasmid

#160686

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP StrepII

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSIQmate

Plasmid

#216240

Purpose

All-in One IQ-Cumate transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term StrepII eGFP 3C

Plasmid

#160683

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

StrepII eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAB1619 hU6-BpiI-Cas13bt1-DR

Plasmid

#176314

Purpose

hU6-BpiI-Cas13bt1-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt1 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAC026

Plasmid

#227598

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

Depositor

Brittany Adamson

Article

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Jmjd3-F

Plasmid

#17440

Purpose

Expression of Jmjd3 in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

JMJD3 (Kdm6b Mouse)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSIQ

Plasmid

#216238

Purpose

All-in One IQ transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR1-SV40-puro

Plasmid

#240535

Purpose

Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-mito

Plasmid

#113919

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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B52_puro_empty_gRNA

Plasmid

#197557

Purpose

sites for cloning two gRNA with puromycin cassette

Depositor

Darius Bägli

Type

Empty backbone

Use

Empty grna backbone with puromycin resistance gene

Expression

Mammalian

Promoter

U6 for gRNA, CMV for puromycin resistance.

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term His8 eGFP 3C

Plasmid

#160684

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-cyto

Plasmid

#113917

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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