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pEBTetD-β-tubulin_SNAP

Plasmid

#136832

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH2

Plasmid

#204230

Purpose

Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH2 (MDH2 Human)

Tags

6X His

Expression

Bacterial

Mutation

Does not include transit peptide and includes a M…

Promoter

Lac

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-MbPylRS

Plasmid

#217361

Purpose

Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. barkeri pyrrolysyl-tRNA synthetase

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-PLA2G15-6xHis WT

Plasmid

#213603

Purpose

Expresses PLA2G15-6xHis in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPyCAG-EGFP-TM-IRES-Pac

Plasmid

#183603

Purpose

Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadCBEd

Plasmid

#193841

Purpose

Express TadCBEd (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEd

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-HypaCas9-mRuby2

Plasmid

#183872

Purpose

pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Type

Empty backbone

Use

CRISPR

Tags

HypaCas9-P2A-mRuby2-T2A-Puro

Expression

Mammalian

Promoter

Cbh

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-POC5

Plasmid

#136786

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-STIL

Plasmid

#136795

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-STIL (STIL Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ-5S-F30-Control

Plasmid

#127582

Purpose

Expresses 5S-F30-Control rRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

5S rRNA with F30 folding scaffold

Expression

Mammalian

Promoter

modified U6

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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mCit(P5f-P13f)3-gs

Plasmid

#228080

Purpose

Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, P5f, P13f

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(P6f)3(P14f)3-gs

Plasmid

#228081

Purpose

Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, P6f, P14f

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
NET29A.Hs pEGFP-N2 (1181)

Plasmid

#62040

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET29A (TMEM120A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Splice variant

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Tags

FLAG, ZZ

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-STIL

Plasmid

#136844

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-STIL (STIL Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-Y207F-V5

Plasmid

#196016

Purpose

Expresses CRKL Y207F, V5 tagged

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCit-APPAP-gs

Plasmid

#228090

Purpose

Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, APPAP

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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eNme2-C TadCBEa

Plasmid

#193840

Purpose

Express TadCBEa (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

eNme2-C TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shTGFBR3 puro

Plasmid

#58696

Purpose

Lentiviral shRNA vector for knockdown of human TGFBR3

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shTGFBR3 (TGFBR3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-Flex-mRuby3-2A

Plasmid

#203846

Purpose

Neuron specific expression of a Cre-dependent mRuby3

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Insert

mRuby3

Use

AAV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRVGP-BB

Plasmid

#15380

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pSBL_DK186

Plasmid

#156422

Purpose

Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein

Tags

EGFP-His

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Myc-SNAP29

Plasmid

#116944

Purpose

Expressing Myc-SNAP29 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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