We narrowed to 1,793 results for: plasmid for e coli
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Plasmid#162670PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
TagsHisExpressionBacterialMutationS131G; codon optimized for expression in E. coli …PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ205
Plasmid#162676PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A anDepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationC224A, C529A; codon optimized for expression in E…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ198
Plasmid#162671PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationF495I; codon optimized for expression in E. coli …PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ196
Plasmid#162669PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225ADepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationS225A; codon optimized for expression in E. coli …PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ204
Plasmid#162677PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationC224H, C529F; codon optimized for expression in E…PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ201
Plasmid#162674PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationC224W, C529SS; codon optimized for expression in …PromoterT7/lacAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
psfaMs-Tn5
Plasmid#183637PurposeExpresses anti-mouse nanobody fused with Tn5DepositorInsertanti mouse secondary nanobody fused to Tn5
Tagsanti-mouse nanobodyExpressionBacterialAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato
Plasmid#60658PurposeFlp-dependent expression of mNaChBac-T2A-tdTomatoDepositorHas ServiceAAV8InsertFLEX-mNaChBac-T2A-tdTomato
UseAAVPromoterEF1αAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato
Plasmid#60660PurposeFlp-dependent expression of mNaChBacMut-T2A-tdTomatoDepositorInsertFLEX-mNaChBacMut-T2A-tdTomato
UseAAVMutationE191KPromoterEF1αAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN4036
Plasmid#74698PurposeOutput plasmid: PPhlF-PAmtR-YFPDepositorInsertPPhlF-PBetI-YFP
ExpressionBacterialAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHis17_ParM _2
Plasmid#78202PurposeExpression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A D63C T174A T175N D224C C287A)
TagsHis6ExpressionBacterialMutationK33A D63C T174A T175N D224C C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CP2-TCC
Plasmid#179679PurposeComplementary plasmidDepositorInsert[sd2] T7 RNAP-TCC linker-degron
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP8045
Plasmid#220900PurposeTn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroidesDepositorInserttnsABCD
ExpressionBacterialAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN4037
Plasmid#74703PurposeOutput plasmid: PPhlF-PBetI-YFPDepositorInsertPPhlF-PBetI-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
ExpressionBacterialPromotermetY native promoterAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1994
Plasmid#139898PurposeExpression plasmid for LetB PLL2 deletion (without TM region)DepositorInsertLetB
ExpressionBacterialMutationLetB(43-877; delta229-239, D228G, L240G)-6xHisAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSELt_S7.A
Plasmid#125835PurposeNegative selection plasmid for evolution of an amber stop codon suppression tRNADepositorInsertACP-2xAmb-pheS
UseSynthetic BiologyExpressionBacterialPromoterpr.trpL*Available SinceMay 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEL1324
Plasmid#139874PurposeExpression plasmid for WT LetB (without TM region) for cryo and negative stain EMDepositorInsertLetB
ExpressionBacterialMutationLetB(43-877)-6xHisAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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His6-TEV-LplA W37V and R337P
Plasmid#248817PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and R337PAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and C55M
Plasmid#248819PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and C55MAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and P167I
Plasmid#248816PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and P167IAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and A48R
Plasmid#248821PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and A48RAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and T57I
Plasmid#248818PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and T57IAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and F147S
Plasmid#248820PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and F147SAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and E265R
Plasmid#248824PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and E265RAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and Y139S
Plasmid#248822PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and Y139SAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and A48N
Plasmid#248823PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and A48NAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V and L172A
Plasmid#248815PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37V and L172AAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA W37V
Plasmid#248814PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialMutationW37VAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-LplA
Plasmid#248813PurposePlasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.DepositorInsertLplA
Tags6xHis-TEVExpressionBacterialAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37U
Plasmid#196659PurposePlasmid for overexpression of mutated initiator tRNA (A37U)DepositorInserttRNAfMet-A37U expression cassette
ExpressionBacterialMutationA37U in tRNAfMetPromotermetY native promoterAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37G
Plasmid#196658PurposePlasmid for overexpression of mutated initiator tRNA (A37G)DepositorInserttRNAfMet-A37G expression cassette
ExpressionBacterialMutationA37G in tRNAfMetPromotermetY native promoterAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only