We narrowed to 8,206 results for: Dos

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pSMP-Dot1L_2

Plasmid

#36340

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Dot1L (DOT1L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-SETDB1_1

Plasmid

#36347

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Bmi1_1

Plasmid

#36352

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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PPDK

Plasmid

#64037

Purpose

Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

Depositor

Vern Schramm

Article

Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

Insert

pyruvate phosphate dikinase

Tags

none

Expression

Bacterial

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
myc-Rapr-FAK

Plasmid

#25926

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pAd5-B5

Plasmid

#122555

Purpose

Block 5 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 17998-25062

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mRFP-ura4MX6

Plasmid

#49183

Purpose

genomic mRFP tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

mRFP

Expression

Yeast

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-kanMX6

Plasmid

#49176

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn(C468A)–GFP

Plasmid

#35959

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Tags

EGFP

Expression

Mammalian

Mutation

c468a

Promoter

cmv

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-FLEX-H2BmTagBFP2-oG

Plasmid

#217976

Purpose

AAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

H2BmTagBFP2
oG

Use

AAV

Tags

mTAGBFP2

Promoter

hSyn

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-SWAP70

Plasmid

#84581

Purpose

Expresses GFP-tagged SWAP70 in mammalian cells

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 (SWAP70 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSMP-MBD3_3

Plasmid

#36373

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD3 (MBD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Suv39H1_2

Plasmid

#36343

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
GFP-TMCC2

Plasmid

#121047

Purpose

Expresses human TMCC2 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pEasyG3_hph

Plasmid

#184919

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUT1159-HQ858703

Plasmid

#27749

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

AUX/IAA transcription factor from DV030103

Use

Gateway Cloning

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pRB051_LEU2

Plasmid

#196993

Purpose

Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

Depositor

Matthew Maurano

Article

Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAd5-B2

Plasmid

#122552

Purpose

Block 2 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 3737-10881

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mg319

Plasmid

#73367

Purpose

unc-70 tension sensor

Depositor

Miriam Goodman

Article

Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

Insert

unc-70::TSMod

Promoter

unc-70p

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn–GFP

Plasmid

#35956

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28c(+)-YTHDF3

Plasmid

#102276

Purpose

For bacterial expression of His-tagged human YTHDF3

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF3 (YTHDF3 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

T7

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMP-EZH1_1

Plasmid

#36359

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH1 (EZH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP-MALAT1M

Plasmid

#120382

Purpose

Reporter for RNA nuclear localization

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

MALAT1 (MALAT1 Human)

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-3xFLAG-G

Plasmid

#127914

Purpose

a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Tags

mNeonGreen-3xFlag

Expression

Mammalian

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDSG312

Plasmid

#115613

Purpose

surface-displayed Nb2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Chimeric_Butterfly_YR_1.0

Plasmid

#59801

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSMP-SUZ12_1

Plasmid

#36390

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SUZ12 (SUZ12 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B3

Plasmid

#122553

Purpose

Block 3 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 10862-14499

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-mCherry-WNT3A

Plasmid

#108345

Purpose

synchronize trafficking of WNT3A from the ER (RUSH System)

Depositor

David Virshup

Article

Moti et al PLoS One. 2019 Feb 22;14(2):e0212711. do

Insert

WNT3A, Wnt family member 3A (WNT3A Human)

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
VV209: sdChR(C138S E154A)-TS-GCaMP6f-ER in fck

Plasmid

#58524

Purpose

Expresses sdChR(C138S E154A) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro-Flag-mAIM2-EGFP

Plasmid

#51667

Purpose

Express Flag and GFP-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP and Flag

Expression

Mammalian

Promoter

MESV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAGmEos2-N1

Plasmid

#46372

Depositor

Suliana Manley

Article

Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

Insert

Gag

Tags

mEos2

Expression

Mammalian

Mutation

Rev independentHXB2 Gag sequence

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJA291

Plasmid

#74487

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

puroR-mCherry-T2A-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-RapR-p38

Plasmid

#25935

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-p38 (Mapk14 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST-attB-yki:V5-S111A, S168A, S250A

Plasmid

#85620

Purpose

Expresses V5 tagged triple mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A,S168,S250A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFC331

Plasmid

#87843

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B6

Plasmid

#122556

Purpose

Block 6 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 25043-30907

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B4

Plasmid

#122554

Purpose

Block 4 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 14475-18018

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTK-DN2

Plasmid

#180283

Purpose

Spacer cassette (TU3)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL2 - Spacer - ConRE

Expression

Bacterial

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG2IG-Hmga1a

Plasmid

#122293

Purpose

Overexpression for mouse Hmga1a

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Hmga1a (Hmga1 Mouse)

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G11-5FLAG

Expression

Yeast

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 8,206 results for: Dos