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We narrowed to 5,937 results for: plasmid dna

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Showing: 1201 - 1250 of 5937 results

  2. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid
    #112157
    Purpose
    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.
    Depositor
    Ben Giepmans
    Article
    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261
    Insert
    anti-GFP FLIPPER-body
    Tags
    His and Thrombin cleavage site
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112157-plasmid-map-sequence-id-213698

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  3. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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  9. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207158-plasmid-map-sequence-id-410280

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  10. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207157-plasmid-map-sequence-id-410282

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  11. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

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  13. pcDNA3-Flag-Tet1 CD Mut

    Plasmid
    #72218
    Purpose
    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 1 (Tet1 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET1 catalytic domain (cata…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3-Flag-Tet2 CD Mut

    Plasmid
    #72220
    Purpose
    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 2 (Tet2 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET2 catalytic domain (cata…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid
    #113962
    Purpose
    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R1 (TAS1R1 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    truncate N-terminal 24 residues
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113962-plasmid-map-sequence-id-223994

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  20. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

    Plasmid
    #157692
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157692-plasmid-map-sequence-id-307834

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  22. pEGFP-C1-DNAJC13(351t)-dimer

    Plasmid
    #246667
    Purpose
    Mammalian expression of truncated, eGFP tagged DNAJC13 (PH-like domain only), with exogenous dimerization system
    Depositor
    Braden Lobingier
    Article
    Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d
    Insert
    DNAJC13 (DNAJC13 Human)
    Tags
    Artificial dimerization domain (Plasmid #124661) …
    Expression
    Mammalian
    Mutation
    Deletion of residues 352 to 2243
    Promoter
    CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246667-plasmid-map-sequence-id-491258

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  28. pcDNA3.3_CoV1_D28

    Plasmid
    #170447
    Purpose
    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    last 28aa deletion in c-terminal tail
    Promoter
    CMV
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170447-plasmid-map-sequence-id-333040

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  44. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

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  45. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

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  46. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

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  47. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  48. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid
    #140978
    Purpose
    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaz-Rluc8 (GNAZ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140978-plasmid-map-sequence-id-300661

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  49. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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  50. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid
    #140975
    Purpose
    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai3-RLuc8 (GNAI3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140975-plasmid-map-sequence-id-300658

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Showing: 1201 - 1250 of 5937 results