We narrowed to 5,097 results for: puc

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pMAL658

Plasmid

#167837

Purpose

Encodes OptoLAC1B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (LAA)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL659

Plasmid

#167838

Purpose

Encodes OptoLAC2B circuit

Depositor

Jose Avalos

Article

Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

Insert

lacI

Use

Synthetic Biology

Tags

ssrA (AAV)

Expression

Bacterial

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA17

Plasmid

#48990

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pNA-Z

Plasmid

#238995

Purpose

Node A carrying sgRNA-2 that can be used for the assembly of pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-2

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-MRscRNA

Plasmid

#240211

Purpose

Yeast vector expressing theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TetO

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MCP-VPR

Plasmid

#240213

Purpose

Mammalian vector for transient expression of MCP-VPR

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

MCP-VPR

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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R26_CAG_BST_FUCCIplex

Plasmid

#240241

Purpose

ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method. The CAG promoter drives the expression of the FUCCIplex sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pNA-Y

Plasmid

#238993

Purpose

Node A carrying sgRNA-1 that can be used for the assembly of pEND-Y11

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-KanR-MCS

Plasmid

#229735

Purpose

For far-red light induced bacterial expression of gene of interest

Depositor

Heikki Takala

Article

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFREDusk-StrR-MCS

Plasmid

#229736

Purpose

For far-red light-induced bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFREDusk-AmpR-MCS

Plasmid

#229737

Purpose

For far-red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Bohm et al ACS Synth Biol. 2025 Jul 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-SynCas.v10 mammalian expression

Plasmid

#208589

Purpose

Expression of SynCas.v10 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v10

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v14A mammalian expression

Plasmid

#208590

Purpose

Expression of SynCas.v14A for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v14A

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-SynCas.v35 mammalian expression

Plasmid

#208591

Purpose

Expression of SynCas.v35 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v35

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v39 mammalian expression

Plasmid

#208592

Purpose

Expression of SynCas.v39 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v39

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SynCas.v41 mammalian expression

Plasmid

#208593

Purpose

Expression of SynCas.v41 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v41

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-SynCas.v47 mammalian expression

Plasmid

#209003

Purpose

Expression of SynCas.v47 for analysis of RNA knockdown

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v47

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

Plasmid

#99696

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

Depositor

George Church

Insert

dCas9 and gRNA targeting Afp (Afp Synthetic)

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP38-1A(sfGFP)

Plasmid

#126984

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-6XDSR

Plasmid

#49865

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-3XDSR

Plasmid

#49863

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-c3HA-spo5DSR

Plasmid

#49872

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-c3HA-6XDSR

Plasmid

#49870

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-c3HA-3XDSR

Plasmid

#49868

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-8XDSR

Plasmid

#49866

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-cyEGFP-4XDSR

Plasmid

#49864

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-c3HA-8XDSR

Plasmid

#49871

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW8ENdeI-c3HA-4XDSR

Plasmid

#49869

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS130

Plasmid

#26290

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-2-PITCh

Plasmid

#63670

Purpose

Expresses Cas9 nuclease and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRPEX18Gm-SceI-pheS

Plasmid

#111609

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pOsU3

Plasmid

#170132

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Expression

Bacterial

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SCP1-dSa-VPR mini.

Plasmid

#99685

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

Plasmid

#179002

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAurora

Plasmid

#190158

Purpose

Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

Depositor

Andreas Moeglich

Article

Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

Type

Empty backbone

Tags

His6 tag

Expression

Bacterial

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

Plasmid

#179008

Purpose

Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA_CAG-mStayGold

Plasmid

#229802

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT_CAG-mRuby2

Plasmid

#229801

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

Plasmid

#179003

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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