We narrowed to 6,187 results for: itch

1. pHR LAT/Zap70 iLID-Drop

   Plasmid

   #171038

   Purpose

   Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-labeled.

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LiOn-CAG∞IRFP

   Plasmid

   #154017

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   IRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CA-Stat3-t2A-mCherry

   Plasmid

   #127540

   Purpose

   Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A662C and N664C. Residues changed to Cys renders …

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. iOn-CAG∞RFP

   Plasmid

   #154014

   Purpose

   Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGV15 (CK2alpha K68M)

   Plasmid

   #27089

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha K68M (kinase dead) (CSNK2A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K68M (kinase dead)

   Promoter

   CMV

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. Sox2-t2A-mCherry

   Plasmid

   #127538

   Purpose

   Expresses human SOX2 and mCherry via t2A linker.

   Depositor

   Jennifer Mitchell

   Article

   Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Actin-mRFP-PAGFP

   Plasmid

   #62382

   Purpose

   Expresses beta actin with both a PAGFP and mRFP tag.

   Depositor

   Guillaume Charras, Tim Mitchison

   Article

   Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

   Insert

   beta actin (ACTB Human)
   

   Use

   Tags

   PAGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64-hERG1a T623A

   Plasmid

   #53055

   Purpose

   Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Threonine 623 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSP64-hERG1a S624A

   Plasmid

   #53056

   Purpose

   Expresses alpha subunit of S624A hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Serine 624 to Alanine

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BRE-MLP

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

11. CMV-NFIB-T2A-miRFP670

    Plasmid

    #187222

    Purpose

    Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Nuclear Factor I B (NFIB Human)
    

    Use

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP18 (CK2alpha' K69M)

    Plasmid

    #27090

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' K69M (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K69M

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. FastLightR-bRaf-mVenus

    Plasmid

    #162155

    Purpose

    Expresses FastLightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    FastLightR-bRaf-mVenus
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

18. LightR-bRaf-mVenus

    Plasmid

    #162154

    Purpose

    Expresses LightR-bRaf-mVenus in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-bRaf-mVenus
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Valine 600 is substituted with Glutamic acid

    Promoter

    CMV

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CK2alpha V66A/I174A-HA (pJD23)

    Plasmid

    #37362

    Depositor

    David Litchfield

    Article

    Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

    Insert

    CK2α V66A/I174A (CSNK2A1 Human)
    

    Use

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    V66A, I174A

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

20. ADGRL3-CTF-Flag

    Plasmid

    #201219

    Purpose

    pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge