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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,400 results for: Coli

Showing: 7471 - 7500 of 9400 results

  3. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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  4. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid
    #122846
    Purpose
    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    human FASN/ human fatty acid synthase (FASN Human)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122846-plasmid-map-sequence-id-238539

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  8. esmGFP-C10-pVTCB052

    Plasmid
    #231303
    Purpose
    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli
    Depositor
    Alexander Rives
    Insert
    esmGFP-C10
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231303-plasmid-map-sequence-id-463619

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  11. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

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  13. pQCascade-array1

    Plasmid
    #140623
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140623-plasmid-map-sequence-id-306003

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  14. pQCascade-IS1

    Plasmid
    #140624
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140624-plasmid-map-sequence-id-305995

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  16. pNCST-AvicFP4

    Plasmid
    #129507
    Purpose
    Expresses AvicFP4 constitutively in E. coli (most strains)
    Depositor
    Nathan Shaner
    Article
    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d
    Insert
    AvicFP4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One of two variants of AvicFP4 tested with essent…
    Promoter
    synthetic constitutive (stationary phase) promote…
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129507-plasmid-map-sequence-id-271859

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  23. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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Showing: 7471 - 7500 of 9400 results