We narrowed to 15,565 results for: LEA

1. pRSFDuet-1_VNp15-DARPinOFF7

   Plasmid

   #182399

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-DARPinOFF7
   

   Use

   Tags

   Vesicle Nucleating peptide 15 (VNp15)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_Pro-IL-36β

   Plasmid

   #214344

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36B (IL36B Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mADAM17deltaMP in pQCXIP cFLAG

   Plasmid

   #19144

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM17 (Adam17 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   delta 189-378

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pGEMT-Map7-1333-end

   Plasmid

   #46070

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1333-end (Map7 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALFY-FYVE 1G

   Plasmid

   #197045

   Purpose

   Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

   Insert

   ALFY (WDFY3 Human)
   

   Use

   Tags

   6xHis-GST-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInsert-PhoCl-RER

   Plasmid

   #219676

   Purpose

   Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   CD4mScarlet-PhoCl-RER
   

   Use

   Tags

   mScarlet and PhoCl

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

    Plasmid

    #52539

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

    Plasmid

    #204975

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-FKBP-mTagBFP2-SOScat

    Plasmid

    #178866

    Purpose

    Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BirA*-HA-NES

    Plasmid

    #214473

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and NES (Nuclear Exclusion Sequence)

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-mCherry.3xFLAG-WPRE

    Plasmid

    #127861

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACEC3GH (Apr)

    Plasmid

    #110085

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-Flag-RIPK1-wt

    Plasmid

    #102677

    Purpose

    retroviral expression plasmid for RIPK1-wt

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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