We narrowed to 15,731 results for: LEA

1. pLVH-EF1A-BRD3R

   Plasmid

   #130696

   Purpose

   Lentiviral plasmids encoding human BRD3R

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   Human BRD3R (BRD3 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21 E2F1

   Plasmid

   #90313

   Purpose

   Expresses human E2F1 in bacteria

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 (E2F1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

   Plasmid

   #134863

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYJ34-PB-CRISPR-BANCR-E1-gRNA

   Plasmid

   #131077

   Purpose

   long non-coding RNA BANCR knock out

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Insert

   BANCR Exon1 gRNA (BANCR Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 HA-rnSOX9 ΔC (1-381)

   Plasmid

   #62973

   Purpose

   Expresses HA-tagged rat SOX9 residues 1-383

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codes for residues 1-381

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-LOx-IRES-EGFP

   Plasmid

   #166877

   Purpose

   Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

   Insert

   Lactate oxidase
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD24-rnc-sfGFP

    Plasmid

    #51560

    Purpose

    Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    rnc (rnc E. coli)
    

    Tags

    GLESTCRHASLAVLADERRFSA and superfolder GFP

    Expression

    Bacterial

    Mutation

    Contains additonal aa at the end of the sfGFP in …

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52545

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attP (full length)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

    Plasmid

    #182826

    Purpose

    Cre-dependent expression of membrane localized APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    LckAPEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRmHA3-CH2F2-CG9866

    Plasmid

    #52286

    Purpose

    Expresses Drosophila Ataxin-7

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    Ataxin-7 (CG9866 Fly)
    

    Tags

    2xHA 2xFLAG

    Expression

    Insect

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. SuExp-mPLD3

    Plasmid

    #201245

    Purpose

    express full length mouse PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (Pld3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIPZ-Myr-PTEN

    Plasmid

    #206983

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    N-terminal myristoylation site

    Expression

    Mammalian

    Promoter

    tet-on promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC292

    Plasmid

    #118881

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    

    Tags

    mRuby3, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    U6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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