We narrowed to 9,193 results for: Pol;

1. pGEX6P1-optimizedNedd4.2d

   Plasmid

   #165103

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

   Insert

   Nedd4.2d (NEDD4L Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Promoter-4xBS_BHLHE40

   Plasmid

   #153064

   Purpose

   Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   4X consensus BHLHE40 binding sites
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTU2-A-RFP (p15A origin)

   Plasmid

   #74090

   Purpose

   pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   R0010 Const. Promoter controlling RFP

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3Bctd-max

   Plasmid

   #198888

   Purpose

   Express A3Bctd-max BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Tags

   6x His and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMW35 p21 (mouse)

   Plasmid

   #16478

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 (Cdkn1a Mouse)
   

   Expression

   Bacterial

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pVETL-Cmcs-Flag-Ataxin3Q25

   Plasmid

   #22118

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLVX Flag RagC WT

   Plasmid

   #112756

   Purpose

   express Flag-RagC

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. -227 hTF

    Plasmid

    #15442

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 GDI2

    Plasmid

    #67093

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GDI2 (GDI2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pLenti CMVie-IRES-BlastR alt MCS

    Plasmid

    #120862

    Purpose

    3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

    Depositor

    Ghassan Mouneimne

    Article

    Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTRY-YFP

    Plasmid

    #15302

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YFP
    

    Use

    Gateway entry

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pbGH

    Plasmid

    #118061

    Purpose

    GBPart - Bovine growth hormone terminator

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    bgH_poly(A)
    

    Use

    Synthetic Biology; Gbpart

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mVenus-PKCB1 K371R

    Plasmid

    #195230

    Purpose

    Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PKCB1 (PRKCB Human)
    

    Use

    Retroviral

    Mutation

    K371R

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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