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pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP

Plasmid

#108506

Purpose

inducible expression of vsv-INCENP 8KQ-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-48-GFP

Plasmid

#108507

Purpose

expression of vsv-INCENP 1-48-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-58-GFP

Plasmid

#108508

Purpose

expression of vsv-INCENP 1-58-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-63-GFP

Plasmid

#108509

Purpose

expression of vsv-INCENP 1-63-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSN29

Plasmid

#100114

Purpose

expresses a chimeric cMYC-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN17

Plasmid

#100104

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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YF-AIP

Plasmid

#61525

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Use

Tags

AIP and EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CF-AIP

Plasmid

#61526

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Use

Tags

AIP and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
L1B39 - pMUTE-Bxb1-tUBQ10

Plasmid

#219704

Purpose

Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase

Depositor

Jennifer Nemhauser

Insert

pMUTE:Bxb1:tUBQ10

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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L1P139 - pSPCH-PhiC31-tUBQ1

Plasmid

#219703

Purpose

Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase

Depositor

Jennifer Nemhauser

Insert

pSPCH:PhiC31:tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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L1P137 - pAHP6-PhiC31-NLS-tUBQ1

Plasmid

#219702

Purpose

Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase

Depositor

Jennifer Nemhauser

Insert

pAHP6:PhiC31-NLS:tUBQ1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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L1B42-pGATA23-Bxb1-SAUR-tUBQ10

Plasmid

#219705

Purpose

Level 1 pGATA23-Bxb1-SAUR-tUBQ10, for lateral root specific expression of Bxb1 integrase with SAUR tag

Depositor

Jennifer Nemhauser

Insert

pGATA23:Bxb1:SAUR:tUBQ10

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/RBD-puro

Plasmid

#161793

Purpose

Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 RBD

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-GFP-IRF3

Plasmid

#127663

Purpose

Over-express EGFP-IRF3

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

IRF3 (IRF3 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

Plasmid

#139089

Purpose

Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

xCas9-NG

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

David Schaffer, Alla Karpova

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Tags

Expression

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/38]

Plasmid

#206777

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/38 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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