We narrowed to 9,193 results for: Pol;

1. pBetaActin-Halo-CAMSAP3

   Plasmid

   #191329

   Purpose

   Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   Halo-CAMSAP3 (Camsap3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NylB'-SCY_pET21b

   Plasmid

   #215417

   Purpose

   Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB'-SCY
   

   Expression

   Bacterial

   Mutation

   R187S/F264C/D370Y

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CAG-Otx2-IRES-GFPd2

   Plasmid

   #73996

   Purpose

   Plasmid expressing Otx2(isoform b)-IRES-GFPd2

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Otx2(isoform b)-Ires-GFP (Otx2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 human cofilin S3A

   Plasmid

   #50854

   Purpose

   expresses constituvely active cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3A (CFL1 Human)
   

   Expression

   Mammalian

   Mutation

   constitutively active, S3A

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET28a-CohIII-CBM-HIS-LPETGG

   Plasmid

   #91697

   Purpose

   CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   CohIII-CBM-HIS-LPETGG
   

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 3XFlag DVL2 (∂DIX)

   Plasmid

   #24805

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   DVL2 (DVL2 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of the N-terminal DIX domain

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. PB-Antares2

   Plasmid

   #120868

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   pcDNA3-Antares2
   

   Expression

   Mammalian

   Mutation

   Antares2 amplicon

   Promoter

   CAG Promoter

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCB169

   Plasmid

   #37390

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   dynein light chain TcTex-type 3 (DYNLT3 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCellFree_G03 IL1B

   Plasmid

   #67066

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL1B (IL1B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pBS hIhh (CT#402)

    Plasmid

    #13995

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Ihh (IHH Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3XFlag DVL2 (∂361-736)

    Plasmid

    #24806

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of aa 361-736

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. C204

    Plasmid

    #174358

    Purpose

    For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIC292

    Plasmid

    #51405

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska2 IMAGE 4685972 (SKA2 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

    Plasmid

    #197968

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pN1-CMV-L-sDarken

    Plasmid

    #184801

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204794

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCellFree_G03 CALR

    Plasmid

    #67086

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CALR (CALR Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTU2-A-RFP (colE1 origin)

    Plasmid

    #74093

    Purpose

    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    R0010 Const. Promoter controlling RFP

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px552-sg-gria1-HT-SEP

    Plasmid

    #187653

    Purpose

    Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    HaloTag-SEP donor
    

    Use

    AAV and CRISPR

    Tags

    N/A

    Promoter

    N/A

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDARMO_CMVT_N_Strep_BIRC6

    Plasmid

    #197972

    Purpose

    Mammalian cell expression of wt Strep-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge