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pC0.286

Plasmid

#119627

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbRChl

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA

Plasmid

#64983

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAG373-mito-redFAST

Plasmid

#160358

Purpose

Expresses mito-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-FKBP-TEVp

Plasmid

#178867

Purpose

Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-FKBP-TEVprotease(Δ220-242)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRK1036

Plasmid

#17728

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB

Plasmid

#178862

Purpose

Mammalian expression of PB1-AzamiGreen-FRB

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB-XIAP1-351

Plasmid

#11560

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 352-496

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
XE70 NLS Beta-catenin GFP in CS2+

Plasmid

#16838

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NLS beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pShGSK3bE

Plasmid

#37526

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

GSK3b (GSK3B Human)

Tags

HA

Expression

Mammalian

Promoter

SRa promoter

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pIVT-osTIR1-9myc

Plasmid

#182043

Purpose

in vitro transcription of osTIR1

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

osTIR1

Use

In vitro transcription

Tags

myc

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pL8_3xG34-DCAF8L2_X1

Plasmid

#191519

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL5_3xG22-DCAF8L2_X1

Plasmid

#191516

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG22-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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(GGS)9_(+36GFP)-Cre.ape

Plasmid

#71747

Purpose

+36-GFP-Cre fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_+36GFP-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
MsCLYBL_sgRNA6

Plasmid

#111295

Purpose

CRISPR KO murine CLYBL

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA6 against murine CLYBL

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBT100.110

Plasmid

#122602

Purpose

clone CDA1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2Y641,645,646F

Plasmid

#22638

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 Y641,645,646F (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Tyrosine 641, 645 and 646 to Phenylalanine

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)MBII85

Plasmid

#67643

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAG374-H2B-greenFAST

Plasmid

#160359

Purpose

Expresses H2B-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

H2B-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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