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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP

Plasmid

#168174

Purpose

Expresses barcoded coding genetic variants in mammalian cells

Depositor

Jesse Boehm, James T. Neal, Aviv Regev

Article

Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wildtype

Promoter

EF-1a

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-PGBD5

Plasmid

#78121

Purpose

Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR2_ccB

Plasmid

#158113

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR2_ccA

Plasmid

#158114

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53 Δ1-42

Plasmid

#196269

Purpose

Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 Δ1-42 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 1 to 42

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_2

Plasmid

#155085

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-EF1-IRES-NEO-Control

Plasmid

#80757

Purpose

Transposon Reporter

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

NeoR (neoR Synthetic)

Promoter

EF1-IRES from PB-EF1-IRES-NEO

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR2_ccC

Plasmid

#158112

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_2

Plasmid

#155099

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PKAsub-TagRFPT-CAAX

Plasmid

#87712

Purpose

Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

PKAsub-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_AAVS1_1801

Plasmid

#159090

Purpose

targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_3

Plasmid

#155089

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccC

Plasmid

#158117

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccA

Plasmid

#158118

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_mouse_Adar2_ccB

Plasmid

#158119

Purpose

deletion mADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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