We narrowed to 10,665 results for: t7

1. pUb-HisUb-Ubc9-ChE1 v4.5

   Plasmid

   #185756

   Purpose

   Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Inserts

   His-Ub
   Ubc9
   ChE1 v4.5
   

   Tags

   His6

   Mutation

   K14R

   Promoter

   T7

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-zfCSF3a

   Plasmid

   #65612

   Purpose

   express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

   Insert

   Csf3a (csf3a Zebrafish)
   

   Tags

   (His)6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 POLD1

   Plasmid

   #67128

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET15b/GgVcl 1-1066 (full length)

   Plasmid

   #46171

   Depositor

   Susan Craig

   Article

   Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

   Insert

   Vcl (VCL Chicken)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-mAIM2-His6

   Plasmid

   #51536

   Purpose

   Express His6-tagged mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH29_32 series plasmid

   Plasmid

   #120901

   Purpose

   Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   Ec-TTL-P109
   sRNA-A targeting sequence
   RBS TIR=974 of GFPop1 gene
   GFPop1
   RBS TIR=6474 of ECF32mut gene
   ECF32mut
   T7 terminator
   resource competitor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 TFAP2B

   Plasmid

   #67092

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAP2B (TFAP2B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBx1-VHH template3-3XMyc-Spacer

   Plasmid

   #176210

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template3-3XMyc-Spacer
   

   Tags

   Myc tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBx1-VHH template2-3XMyc-Spacer

   Plasmid

   #176209

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template2-3XMyc-Spacer
   

   Tags

   Myc tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCSG7-D16A-NmeCas9

    Plasmid

    #71476

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-D16A-NmeCas9
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sc eRF1 (WT)

    Plasmid

    #175413

    Purpose

    Encodes His-MBP-TEV-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_TIAL1

    Plasmid

    #106092

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIAL1 (TIAL1 Human)
    

    Tags

    6xHis-thrombin and T7

    Expression

    Bacterial

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-P.accABCD

    Plasmid

    #66866

    Purpose

    Encodes accA, accB, accC, accD in Pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    accA
    accB
    accC
    accD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b-dspB(E184Q)-6xHis

    Plasmid

    #175802

    Purpose

    Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHisIHFαIHFβ

    Plasmid

    #149384

    Purpose

    Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Inserts

    Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
    Pseudomonas aeruginosa PA14 IHFbeta
    

    Tags

    6xHis tag and HRV3C cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB036d

    Plasmid

    #99216

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and PproA

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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