We narrowed to 22,191 results for: his

1. HOXD12 (murine) pRSETa

   Plasmid

   #8554

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXD12 (Hoxd12 Mouse)
   

   Use

   Tags

   HIS and XPress

   Expression

   Bacterial

   Mutation

   Fusion sequence is: ggatccATG

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pDDGFP-Leu2d_GgPCFT

   Plasmid

   #165414

   Purpose

   expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

   Insert

   GgPCFT (SLC46A1 Synthetic, Chicken)
   

   Use

   Tags

   GFP-His8

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. K339CLM G234A/215/347

   Plasmid

   #24449

   Depositor

   Ron Vale

   Article

   Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

   Insert

   Kinesin (KIF5B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E…

   Promoter

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCellFree_G03 CSNK2A1

   Plasmid

   #67085

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CSNK2A1 (CSNK2A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pRSET-spa-GCaMP6f

   Plasmid

   #67563

   Purpose

   bacterial expression of a photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6f
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. HOXA9 (murine) FLAG pET

   Plasmid

   #8494

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA9 (Hoxa9 Mouse)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   FLAG epitope tag replaces T7 tag in the pET back…

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET15b-At 14-3-3 nu

   Plasmid

   #44940

   Depositor

   Robert Ferl

   Article

   Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

   Insert

   14-3-3 nu (GRF7 Mustard Weed)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. D92_Ld_360170

   Plasmid

   #135905

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA-sfGFP

   Plasmid

   #162689

   Purpose

   pFA vector control expressing sfGFP

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   sfGFP
   

   Use

   Tags

   C terminal 6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   PL tet promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LacD.1_WT_pET21a

    Plasmid

    #29789

    Depositor

    SeWon Suh

    Article

    Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

    Insert

    Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pQTEV-MPP6

    Plasmid

    #34806

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    membrane protein, palmitoylated 6 (PALS2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFS217-USP44_MSCV-TAP

    Plasmid

    #43896

    Purpose

    Retroviral expression of USP44 in mammalian cells

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Retroviral

    Tags

    FLAG, HA, and HIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-NEMOL329P

    Plasmid

    #11969

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine329 to Proline

    Promoter

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

15. K347CLM G234A/43/347

    Plasmid

    #24454

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

    Promoter

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCellFree_G03 SMAD2

    Plasmid

    #67095

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA6-rbN1 S77A

    Plasmid

    #32945

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Change Serine77 to Alanine

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFR092

    Plasmid

    #134148

    Purpose

    Expression of CENP-T

    Depositor

    Iain Cheeseman

    Article

    Nishino et al EMBO J. 2013 Feb 6;32(3):424-36. doi: 10

    Insert

    CENP-T (CENPT Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-375

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 TYK2

    Plasmid

    #67133

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TYK2 (TYK2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    A435S

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pCellFree_G03 GTF2H4

    Plasmid

    #67094

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H4 (GTF2H4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pCellFree_G03 MAPK13

    Plasmid

    #67077

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    MAPK13 (MAPK13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pANY1

    Plasmid

    #110949

    Purpose

    Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

    Insert

    ccd B cassette
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA6-rbN1 S77D

    Plasmid

    #32947

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    slc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Serine 77 mutated to Aspartic Acid

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-N1-PDZ1-GAGA

    Plasmid

    #31651

    Depositor

    Edward Weinman

    Article

    Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

    Insert

    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
    

    Use

    Tags

    His, S-tag

    Expression

    Mammalian

    Mutation

    changed P(21)NGYGF changed to H(21)NGAGA

    Promoter

    cmv

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-N1-PDZ2-GAGA

    Plasmid

    #32857

    Depositor

    Edward Weinman

    Article

    Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

    Insert

    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)
    

    Use

    Tags

    His,S-tag

    Expression

    Mammalian

    Mutation

    PDZ2 P(161)NGYGF mutated to H(161)NGAGA

    Promoter

    cmv

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PrbC10

    Plasmid

    #24788

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    Prb
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

    Promoter

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

29. spa-GCaMP6m

    Plasmid

    #67557

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6m
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCellFree_G03 ATF3

    Plasmid

    #67057

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    M2I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGB1-mCRT(P-domain)

    Plasmid

    #83519

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    P domain (contains residues 187-283)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pGB1-mCRT(C-domain)

    Plasmid

    #83518

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Use

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    C domain (contains residues 340-399)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pET16b-Trx80

    Plasmid

    #36945

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Trx80 (truncated Trx) (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    premature stop inserted after K80 in Trx1 gene

    Promoter

    T7

    Available Since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

35. Human Mevalonate diphosphate decarboxylase (MDD)

    Plasmid

    #52431

    Purpose

    bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

    Depositor

    Henry Miziorko

    Article

    Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

    Insert

    mevalonate diphosphate decarboxylase (MVD Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

36. PH1109

    Plasmid

    #24830

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    PH1109 (PH1109 Pyrococcus horikoshii)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

37. PA1024_pET21a(+)C-tag

    Plasmid

    #14579

    Depositor

    SeWon Suh

    Article

    Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

    Insert

    2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pET50delCT_Vipp1_H1-6

    Plasmid

    #223816

    Purpose

    Bacterial expression of Vipp1 H1-6 with C-terminal 6x His-Tag and 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

    Insert

    Vipp1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    truncation of helix 0

    Promoter

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. IKKε v2-V5

    Plasmid

    #169770

    Purpose

    Express IKKε v2 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2
    

    Use

    Tags

    V5 tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSUMO1-del

    Plasmid

    #228950

    Purpose

    express the full-length human SAE1:SAE2 heterodimer ( where SAE1 is untagged and SAE2 contains an N-terminal tag : MGSSHHHHHHSQDADLNSRVD, derivated from the Addgene plasmid #52258 pSUMO1)

    Depositor

    Marcin Suskiewicz

    Inserts

    UBA 2 (UBA2 Human)
    Aos1 (SAE1 Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGSSHHHHHHS…

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7-lac

    Available Since

    May 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge