We narrowed to 14,422 results for: NTS

1. pFH71

   Plasmid

   #128214

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUPI AoT5Cyp

   Plasmid

   #80280

   Purpose

   Expresses Aotus TRIM5 CypA fusion

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   TRIM5 CypA fusion
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_tAKARζ

   Plasmid

   #119918

   Purpose

   Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARζ
   

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR2KN0079

   Plasmid

   #117646

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-1459-adcy4-Luc

   Plasmid

   #28184

   Depositor

   Bernard Schimmer

   Article

   Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

   Insert

   Adcy4 promoter (Adcy4 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

6. AAV-oChD-citrine

   Plasmid

   #50976

   Purpose

   AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   oChD-citrine
   

   Use

   AAV

   Tags

   citrine

   Mutation

   ChD gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CP1300-CBEmax

   Plasmid

   #119805

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   rApobec1-nSpCas9 (CP1300) -UGI-UGI
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT mir-1-1 reporter cel-mir-40

   Plasmid

   #46739

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   cel-mir-40
   

   Use

   RNAi

   Tags

   His and hsa-mir-1-1

   Expression

   Insect

   Promoter

   Metallothionein (MT)

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-SZT2 1-1021

   Plasmid

   #87037

   Purpose

   mammalian expression of SZT2 fragment

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AA 1-1021

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Mutation

    K810A, K1003A, R1060A

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS ZfHoxd3a

    Plasmid

    #27534

    Depositor

    Victoria Prince

    Article

    Prince et al Development. 1998 Feb . 125(3):393-406.

    Insert

    Hoxd3a (hoxd3a Zebrafish)
    

    Use

    Cloning vector

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pDIRECT_37J

    Plasmid

    #91153

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:TALEN
    

    Use

    TALEN

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PI(DM)-Rac1(WT)dc1.FLARE

    Plasmid

    #87589

    Purpose

    PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    PBD
    Rac1
    

    Tags

    YPet and dTurq

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHJ32

    Plasmid

    #48361

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_A1310

    Plasmid

    #91034

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A nickase
    

    Use

    CRISPR

    Mutation

    H840A

    Promoter

    ZmUbi

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

    Plasmid

    #68274

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_A0208

    Plasmid

    #91004

    Purpose

    Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A nickase
    

    Use

    CRISPR

    Mutation

    D10A

    Promoter

    EC1.2

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FusionRed-CAF1-C-10

    Plasmid

    #56102

    Purpose

    Localization: Nucleus, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CAF1
    

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBABE-zeo small T C97S E102Q

    Plasmid

    #9064

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    SV40 small T Antigen 97, 102 (SV40gp7 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    C97S, E102Q

    Available Since

    Sept. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pBS Rootletin (Nigg pFL1(CW498))

    Plasmid

    #41168

    Depositor

    Erich Nigg

    Article

    Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

    Insert

    Rootletin (CROCC Human)
    

    Expression

    Bacterial

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only