59,541 results

1. SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

   Plasmid

   #170482

   Purpose

   Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

   Depositor

   Dirk Heckl

   Article

   Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

   Inserts

   dCas9-KRAB
   SFFV
   P2A-mNeonGreen
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-Ef1a-SOX2-3xFLAG

   Plasmid

   #216197

   Purpose

   Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SRP19 in pLX311

   Plasmid

   #233292

   Purpose

   Expression of WT SRP19

   Depositor

   Sefi Rosenbluh

   Article

   Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

   Insert

   SRP19 (SRP19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mStayGold-ACTB

   Plasmid

   #227326

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-mStayGold-TUBA1B

   Plasmid

   #227321

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FFA2-DuET

   Plasmid

   #213238

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   FFA2 (FFAR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

   Plasmid

   #210709

   Purpose

   This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Kif5b-T92N-Myc

   Plasmid

   #186615

   Purpose

   Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   T92N

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-XPO5-His6

   Plasmid

   #171562

   Purpose

   Encodes human XPO5 protein

   Depositor

   Yan Zeng

   Insert

   XPO5 (XPO5 Human)
   

   Expression

   Bacterial

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-ET-F656W

    Plasmid

    #72546

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F656W

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-ET-I654Q

    Plasmid

    #72545

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I654Q

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-ET-L630W

    Plasmid

    #72543

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L630W

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CDK9 gRNA (BRDN0001162576)

    Plasmid

    #77170

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK9

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK9 (CDK9 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK449 (BromoTag-mClover-BSD)

    Plasmid

    #214379

    Purpose

    BromoTag-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mClover-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK464 (mAB-Neo)

    Plasmid

    #214390

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BromoTag-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-23:TJP1-mEGFP

    Plasmid

    #87429

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. GFP-rab9 WT

    Plasmid

    #12663

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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