We narrowed to 27,126 results for: STI

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SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLX311 hcRed1

Plasmid

#117733

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-DIO-CB2-EGFP

Plasmid

#91946

Purpose

Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

Depositor

Jimok Kim

Article

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Inserts

EGFP
type-2 cannabinoid receptor

Use

AAV

Tags

FLAG and None

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP-mTLN1-FL

Plasmid

#164838

Purpose

Expression of GFP tagged FL mouse talin1

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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p182 pK7-HDAC1 mut (GFP)

Plasmid

#11055

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-56: deacetylase domain deletion

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-T-Sapphire

Plasmid

#233209

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vinculin_TurboID_Venus

Plasmid

#200272

Purpose

Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Expression

Mammalian

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk5-DN

Plasmid

#1871

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk5 (CDK5 Human)

Expression

Mammalian

Mutation

D145N, dominant negative

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pUCmini-UGCG-tdTomato

Plasmid

#225879

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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YTIP-GFP

Plasmid

#42238

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YTIP-GFP

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-GSK3B_IDG-K

Plasmid

#158164

Purpose

Gateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

TurboID-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSW457 - tCLV3

Plasmid

#115980

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSW455 - pCLV3

Plasmid

#115979

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCLV3

Use

Unspecified

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p667-UBC-AAK1-V5_IDG-K

Plasmid

#135257

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5 (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGrDL_SP

Plasmid

#83204

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

SalI/PstI cloning sites

Expression

Plant

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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IQ013: pMVP/Ad-DEST

Plasmid

#121843

Purpose

pMVP destination vector, empty adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK332

Plasmid

#59573

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX3x-hRanBP2-(IR1+M)

Plasmid

#53140

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2631-2711)

Promoter

tac

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pVL26 - pSCR

Plasmid

#116021

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSCR

Use

Unspecified

Mutation

T171A by SDM to abolish Eco31I site

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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ZE45 Dvl-2 pCS2P+

Plasmid

#17086

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dvl-2 (dvl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pHL 246

Plasmid

#53014

Purpose

AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

GFP

Use

Synthetic Biology

Tags

fimS (off orientation)

Expression

Bacterial

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pLK69

Plasmid

#37258

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

lacks the 4 C-term aa

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
GFP-Myo 1g

Plasmid

#134991

Purpose

Expression of mouse Myo 1g in mammalian cells.

Depositor

Martin Bähler

Article

Olety et al FEBS Lett. 2010 Feb 5;584(3):493-9. doi:

Insert

Myo 1g (Myo1g Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Insert

PCP-NLS-GFPEnvy

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.TfrOVA

Plasmid

#69596

Purpose

contains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-mOVA mice)

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5-myc-Erk2

Plasmid

#39222

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2001 Jul 13;276(28):26509-1

Insert

Erk2 (Mapk1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2013

Availability

Academic Institutions and Nonprofits only
SpRY

Plasmid

#198544

Purpose

Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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