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We narrowed to 18,759 results for: nls

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Showing: 10081 - 10120 of 18759 results

  1. PX458_ZNF219_2

    Plasmid
    #72369
    Purpose
    Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF219 (ZNF219 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72369-plasmid-map-sequence-id-134663

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  2. PX458_SSRP1_1

    Plasmid
    #72371
    Purpose
    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSRP1 (SSRP1 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72371-plasmid-map-sequence-id-132121

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  9. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN exon 5 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

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  13. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  15. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid
    #68330
    Purpose
    Gluconacetobacter diazotrophicus Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68330-plasmid-map-sequence-id-119553

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  16. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Publication
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

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  18. YFP(159-238)-gamma-1 in pcDNAI/Amp

    Plasmid
    #54471
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(159-238)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54471-plasmid-map-sequence-id-115002

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  23. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  24. pJZC73

    Plasmid
    #62341
    Purpose
    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62341-plasmid-map-sequence-id-102264

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  27. pGfa28-nLac

    Plasmid
    #53130
    Purpose
    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.
    Depositor
    Michael Brenner
    Publication
    Lee et al Glia. 2006 May;53(7):677-87.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains the A and B regions (bp -1757 to -1489) …
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53130-plasmid-map-sequence-id-82730

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  28. pGfa(B3)-nLac

    Plasmid
    #53132
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Publication
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of the gfa2 B segment…
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53132-plasmid-map-sequence-id-82721

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  30. pTALEN(Nd154C63) Ef1a

    Plasmid
    #50433
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50433-plasmid-map-sequence-id-75354

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  39. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35188-plasmid-map-sequence-id-42839

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  40. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    40788-plasmid-map-sequence-id-68904

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Showing: 10081 - 10120 of 18759 results