We narrowed to 18,890 results for: Tec

1. pCfB6681

   Plasmid

   #106157

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 PSMC1

   Plasmid

   #67087

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PSMC1 (PSMC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   E275G, I287V and R294G

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

   Plasmid

   #141025

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   EGFP
   

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-L1-PGK-Neo-L2

   Plasmid

   #141007

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Expression

   Mammalian

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH1-SaSrtA-KA

   Plasmid

   #64981

   Purpose

   Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108A

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEU3-NII-HLICNot

   Plasmid

   #140181

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   pEU3-NII-HLICNot

   Promoter

   T7

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2275

   Plasmid

   #36712

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MDM2-TALEN_Right (Mdm2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. TAL2400

   Plasmid

   #36803

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC6-TALEN_Left (Hdac6 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMB1-spect-PthrC3-eGFP

   Plasmid

   #107409

   Purpose

   This plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCKan-yibDp-mdlC

    Plasmid

    #134590

    Purpose

    Low Phosphate inducible mdlC benzylformate decarboxylase

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mdlC (benzylformate decarboxylase) C -terminal his tag
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAL2296

    Plasmid

    #36729

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BAX-TALEN_Left (Bax Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TAL2297

    Plasmid

    #36730

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BAX-TALEN_Right (BAX Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. TAL2328

    Plasmid

    #36754

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MLH1-TALEN_Left (Mlh1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TAL2329

    Plasmid

    #36755

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MLH1-TALEN_Right (Mlh1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL2413

    Plasmid

    #36814

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BCL6-TALEN_Right (Bcl6 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pDisplay FLIPE-100u

    Plasmid

    #13547

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL2414

    Plasmid

    #36815

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    EXT1-TALEN_Left (Ext1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. TAL2361

    Plasmid

    #36774

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CBX8-TALEN_Right (Cbx8 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL1-PorA-4xDQNAT

    Plasmid

    #128392

    Purpose

    In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    PorA
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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