We narrowed to 5,577 results for: dre

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Promoter

T7

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir2-LNS2

Plasmid

#220086

Purpose

C-terminal PA binding domain of Nir2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM1 (PITPNM1 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 896-1216

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL2-pCMV7.1

Plasmid

#155020

Purpose

N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 2 (CUL2 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSICO-eGFP-TNPO3

Plasmid

#167590

Purpose

Expresses human TNPO3 fused to eGFP

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

eGFP-TNPO3 (TNPO3 Human)

Use

Lentiviral

Tags

eGFP

Mutation

WT

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma2

Plasmid

#165495

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL7-pCMV7.1

Plasmid

#155025

Purpose

N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 7 (CUL7 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDawn-Ag43

Plasmid

#107742

Purpose

Expresses Ag43 under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAS9id

Plasmid

#141251

Purpose

Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEHIShqprt

Plasmid

#92166

Purpose

Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

Quinolinate Phosphoribosyltransferase (QPRT Human)

Tags

TEV protease -cleavable N-terminal 6His

Expression

Bacterial

Mutation

changed Asp 2 to Asn to create BspHI site

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(HA)

Plasmid

#195022

Purpose

Gateway vector containing HA-tagged Creb5

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mLmx1a

Plasmid

#33013

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-G

Plasmid

#216115

Purpose

Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

oROS-G

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr_LF(C199S)

Plasmid

#216114

Purpose

Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

oROS-Gr_LF(C199S)

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

Plasmid

#156463

Purpose

Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

Depositor

Andre Stiel

Article

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

Heme Oxygenase
ReBphP-PCM

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSLGCV

Plasmid

#49862

Purpose

Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

Depositor

Anne Glover, Jonathan Pettitt

Article

Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

Insert

firefly luciferase luc+ fused to GFP (S65C)

Tags

GFP (S65C)

Expression

Worm

Promoter

sur-5

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

V5-His

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 BARK1 minigene

Plasmid

#14695

Depositor

Robert Lefkowitz

Article

Koch et al J Biol Chem. 1994 Feb 25. 269(8):6193-7.

Insert

BARK1 minigene (GRK2 Bovine)

Tags

3'UTR of human b-globin

Expression

Mammalian

Mutation

BARK1 amino acids 495-689

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pFastBac_His-ZZ-TEV-Lis1

Plasmid

#132539

Purpose

Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

Depositor

Andrew Carter

Article

Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

Insert

Lis1 (PAFAH1B1 Human)

Tags

8x His, Linker-TEV-linker_TEV, and ZZ

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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