164,826 results

1. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-SMARCA4

   Plasmid

   #65391

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. alpha7nACHR_Nacho_mCherry

   Plasmid

   #182939

   Purpose

   alpha7 nACHR expression in mammalian cell lines

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Synthesized

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. spMSP1D1

   Plasmid

   #173482

   Purpose

   Expresses spMSP1D1 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spMSP1D1
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM461

   Plasmid

   #108320

   Purpose

   For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   Che9C RecT
   Bxb1 Integrase
   Tet Repressor
   SacRB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti F9-TetR-EGFP-IRES-PuroR

   Plasmid

   #117049

   Purpose

   lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Insert

   F9-TetR-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   F9 promoter

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVTNT-T7-KPNA2

   Plasmid

   #26678

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 2 (KPNA2 Human)
   

   Use

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACS141

    Plasmid

    #204597

    Purpose

    Histone H3.3-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human histone H3.3 (H3-3A Human)
    

    Use

    Tags

    human histone H3.3 - V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TrxRFP2

    Plasmid

    #172322

    Purpose

    an improved fluorescent biosensor for thioredoxin redox

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    TrxRFP2, an improved version from TrxRFP1
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR CD19-FcGamma CAR

    Plasmid

    #113014

    Purpose

    for production of lentivirus encoding the CD19 CAR-Pfcgamma

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P intracellular GFP (CD19 Synthetic, Human, Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi G418R mbIL21

    Plasmid

    #194329

    Purpose

    Consitiutive mammalian expression of membrane bound human IL-21

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    mbIL-21 (IL21 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_BiPVe4_ChR2_mCherry

    Plasmid

    #213937

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE-8XGTIIC-DsRED-DD

    Plasmid

    #115798

    Purpose

    lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP responsive promoter (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    DsRED-DHFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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