162,160 results

1. pcDNA3.1-HA-mUbp43

   Plasmid

   #12454

   Depositor

   Dong-Er Zhang

   Article

   Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

   Insert

   UBP43 (Usp18 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCnRed

   Plasmid

   #232908

   Purpose

   Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   gam (gam phage Lambda, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Pbad-rha

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRC_RR_VP1_r1c3

   Plasmid

   #65724

   Purpose

   Rescued infectious variant from pAAV2_RaPID library; mCherry insertion in place of the deleted amino acid sequence 453-GTTTQSR

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. IL6R PLAK1

   Plasmid

   #161518

   Purpose

   Expresses IL6 receptor (full length) in mammalian cells.

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   Interleukin 6 Receptor (IL6R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF0500

   Plasmid

   #143709

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARGC1A (PPARGC1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197065

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   I3-01
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LeGO-V2

   Plasmid

   #27340

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, Venus fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-Klf4

   Plasmid

   #13370

   Purpose

   Retroviral expression of mouse Klf4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6.3/TO/CD9-GFP

   Plasmid

   #104402

   Purpose

   Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

   Depositor

   Jens Gruber

   Article

   Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

   Insert

   CD9 (CD9 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid

    #188375

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNP568

    Plasmid

    #222725

    Purpose

    pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

    Plasmid

    #200641

    Purpose

    Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

    Depositor

    Fleur Ferguson, Justin English

    Article

    Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

    Insert

    miniTurboID-6xGGSG linker-FKBPF36V-2xHA
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-CMV-H-sDarken

    Plasmid

    #184800

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV-CAG-FLEX-BFP2-(mCherry)’

    Plasmid

    #115234

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCas9-CR4

    Plasmid

    #62655

    Purpose

    Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    Cas9 nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-TRE3g-mNeon-WPRE-pA

    Plasmid

    #192064

    Purpose

    mNeonGreen expression with TRE3g promoter

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3g

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-LiLac

    Plasmid

    #184570

    Purpose

    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

    Depositor

    Gary Yellen

    Article

    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

    Insert

    LiLac fluorescent sensor of lactate
    

    Use

    AAV

    Tags

    His7

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. qTAG-N-Puro-mStayGold

    Plasmid

    #227250

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-alpha4 wt

    Plasmid

    #80016

    Purpose

    Mammalian expression of human integrin alpha4

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    integrin alpha4 (ITGA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

28. GFP-C1-AKT-PH

    Plasmid

    #21218

    Depositor

    Tobias Meyer

    Article

    Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

    Insert

    AKT-PH
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of AKT. (Lab plasmid WC 0002).

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge