We narrowed to 40,266 results for: KAN

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pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSD103D

Plasmid

#218134

Purpose

For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD55

Tags

MBP

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJM189A

Plasmid

#218135

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSLC-238

Plasmid

#73192

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-chpB (chpB )

Expression

Bacterial

Promoter

PrhaB

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pMM0646

Plasmid

#129007

Purpose

Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1-EGFP

Plasmid

#62604

Purpose

Constitutive expression of FPR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

Insert

FPR1 (FPR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCHC039 (∆phcA)

Plasmid

#226207

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pMpGWB215

Plasmid

#68606

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pTMEM-PhoCl-Insert

Plasmid

#219674

Purpose

Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

TMEM-PhoCl-rtTA3

Tags

PhoCl

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Tom20-mCherry-GAI(1-92)

Plasmid

#37316

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

Tom20 and mCherry

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pHS0663 pET45b(+) KraIscB-1 TwinStrep

Plasmid

#176536

Purpose

Bacterial expression of KraIscB-1-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

KraIscB-1

Tags

TwinStrep

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM55297

Plasmid

#153213

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC2-UMPS-3xFLAG

Plasmid

#87975

Purpose

3rd generation lentiviral vector for Expression of UMPS in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71346

Purpose

Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern(Ca2+)

Expression

Mammalian

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pMpGWB405

Plasmid

#68670

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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