We narrowed to 7,952 results for: Lif
-
Plasmid#190680PurposeEncodes an EGFRvIII-CD3 BITE protein (F263 clone)DepositorInsertAnti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_LamB1
Plasmid#181999PurposeCMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell linesDepositorInsertSTf-LamB1
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (Q70L)
Plasmid#184037PurposeExpresses constitutively active mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
TagsmCherryExpressionMammalianMutationGlutamine 70 changed to LeucinePromoterCMV IE94Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-CAG-GFP-EnvA
Plasmid#102987PurposeLentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted RabiesDepositorInsertsEnhanced Green Fluorescent Protein (eGFP)
EnvA-B19 Rabies Glycoprotein
UseLentiviralAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
STOP-eGFP-ROSA26TV
Plasmid#11739DepositorInsertSTOP-eGFP-ROSA26TV
UseCre/Lox; /stopAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Rab11a (S25N)
Plasmid#184036PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryosDepositorInsertRab11
TagsmCherryExpressionMammalianMutationSerine 25 changed to AsparaginePromoterCMV IE94Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138129PurposeExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmClover3-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-OPTN(D474N)
Plasmid#119683PurposeFor mammalian expression of UBD mutant OPTNDepositorAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F265-OKT3
Plasmid#190679PurposeEncodes an EGFRvIII-CD3 BITE protein (F265 clone)DepositorInsertAnti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-EGFP-RIP2
Plasmid#131201Purposemammalian expression, tet RDepositorAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-B
Plasmid#177785PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_TOMM20-STf
Plasmid#182002PurposeCMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell linesDepositorInsertTOMM20-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSup_35Senh(fwd)_35Spr+synJ
Plasmid#149422PurposePlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.DepositorInsert35S core enhancer
ExpressionPlantAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NET29A.Hs pEGFP-N2 (1181)
Plasmid#62040Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1a
Plasmid#112221Purposemammalian expression of flag tagged GREB1aDepositorAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
C413-E45: attB4-hr5-ie1p>-attB1r
Plasmid#162930PurposeGateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPVDepositorInsertAcMNPV ie1 promoter with hr5 enhancer
UseSynthetic BiologyAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only