We narrowed to 5,895 results for: SPI

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pEB1N-mApple-LZ-LOV2

Plasmid

#197418

Purpose

Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

Insert

MAPRE1 (MAPRE1 Human)

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R363L

Plasmid

#180381

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R363L (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R363L

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_D35N_R49Q

Plasmid

#180383

Purpose

lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_D35N/R49Q (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

D35N/R49Q

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-3

Plasmid

#180390

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-3 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-2

Plasmid

#180389

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-2 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-1

Plasmid

#180388

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-1 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ285 (Lb5Cas12a)

Plasmid

#138120

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1ΔN

Plasmid

#213508

Purpose

Expresses human eGFP-SUNΔN in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

eGFP-SUNΔN

Expression

Mammalian

Mutation

SUN1ΔN has the first 138 amino acid (lamina domai…

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2

Plasmid

#115192

Purpose

Lentiviral transduction and expression of PDHA2 into any mammalian cell

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PDHA2WT (PDHA2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

Plasmid

#197063

Purpose

Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Inserts

Encapsulin
Adaptor Protein

Use

CRISPR

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

Plasmid

#185910

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpCas9-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A

Promoter

CMV and T7

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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2XSmBiT-SARS-CoV-2 S1

Plasmid

#173430

Purpose

protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)

Use

Luciferase

Tags

human IgG1 Fc

Expression

Mammalian

Mutation

furin cleavage site RRAR to GSAS

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-Mbd3

Plasmid

#52356

Purpose

Lentiviral expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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