We narrowed to 5,822 results for: pCas

1. pIR197

   Plasmid

   #64819

   Purpose

   BiFC rCUC2

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

   Insert

   CUC2 (CUC2 Mustard Weed)
   

   Tags

   C-terminal domain Firefly

   Mutation

   rCUC2

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. hs-FL-Kek1-myc2

   Plasmid

   #41170

   Depositor

   Norbert Perrimon

   Article

   Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

   Insert

   Kek1 (kek1 Fly)
   

   Tags

   2X myc

   Expression

   Insect

   Promoter

   Hsp70

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

3. D-raf G621S

   Plasmid

   #39769

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Expression

   Insect

   Mutation

   G621S

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

4. D-raf C295S

   Plasmid

   #39768

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Expression

   Insect

   Mutation

   C295S

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

5. D-raf F290I

   Plasmid

   #39766

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Expression

   Insect

   Mutation

   F290I

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

6. D-raf R217L+S620A

   Plasmid

   #39765

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Expression

   Insect

   Mutation

   R217L and S620A

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

7. D-raf R217L+C295S

   Plasmid

   #39764

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Expression

   Insect

   Mutation

   R217L and C295S

   Available Since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXN2-BCL6 (CXN-BCL6)

   Plasmid

   #40346

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   BCL6 (BCL6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_PE7-2A-BSD

    Plasmid

    #234073

    Purpose

    For lentiviral expression of PE7 with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PE7
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHSE401

    Plasmid

    #62201

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Article

    Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Expression

    Bacterial

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHeACT-mEGFP-NLS

    Plasmid

    #205003

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYL156 (TRV RNA2)

    Plasmid

    #148969

    Purpose

    Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    Modified TRV RNA2
    

    Use

    T-dna vector

    Mutation

    see comments section below

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR1.cas9-NT

    Plasmid

    #197871

    Purpose

    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

    Depositor

    Baojun Wang

    Article

    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

    Inserts

    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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