162,682 results

1. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PDE4D2cat-ICUE4

   Plasmid

   #181847

   Purpose

   ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
   

   Use

   Tags

   ECFP, PDE4D2(86-418), and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX305_3xFLAG_STAT1

   Plasmid

   #220323

   Purpose

   Expresses full length human STAT1 with an N-terminal 3xFLAG tag

   Depositor

   Jonathan Brown

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   PGK

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MIB2-FLAG

   Plasmid

   #37117

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGENT R2 GW R1 RUBY

   Plasmid

   #225988

   Purpose

   To express RUBY transgene under the control of desired promoter (Note: R2 Gateway R1)

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   Tags

   RUBY

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCPP5372

   Plasmid

   #128733

   Purpose

   Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

   Depositor

   Alan Collmer

   Article

   Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. E-cadherin-GFP

   Plasmid

   #28009

   Depositor

   Jennifer Stow

   Article

   Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

   Insert

   E-cadherin (CDH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCS-mCyRFP1

    Plasmid

    #84545

    Purpose

    Bacterial expression of mCyRFP1

    Depositor

    Michael Lin

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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