We narrowed to 16,014 results for: Por

1. pTXTL-T7max-FLuc

   Plasmid

   #188311

   Purpose

   Expression of Firefly luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Firefly luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-MinE-EGFP_IRES-Blast

   Plasmid

   #213113

   Purpose

   Expression of MinE-EGFP in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKO2-pBAD

   Plasmid

   #54555

   Purpose

   Localization: Protein Expression Vector , Excitation: 551, Emission: 565

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mKO2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

   Plasmid

   #130816

   Purpose

   Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   bJun-NFAST-NLS-DEVDG-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-Mycn

   Plasmid

   #60667

   Purpose

   PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Mycn (Mycn Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205551

   Purpose

   Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-I
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK467 (dTAG-BromoTag-mAID-EGFP)

   Plasmid

   #214393

   Purpose

   dTAG-BromoTag-mAID-EGFP reporter (TOL2)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   dTAG-BromoTag-mAID-EGFP reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET20b-NLuc-MBP-6xHis

   Plasmid

   #135931

   Purpose

   Expresses NanoLuciferase in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAM1.17_PB-STEAP3-PGK-Blast

   Plasmid

   #205562

   Purpose

   Express human STEAP3

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   STEAP3 (STEAP3 Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

    Plasmid

    #214994

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-eNOS-GFP

    Plasmid

    #22444

    Depositor

    William Sessa

    Article

    Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

    Insert

    WT eNOS-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC-6-ZmBbm-P2A-ZmWus2-9

    Plasmid

    #197750

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmBbm-P2A-ZmWus2 bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5_tAKARβ

    Plasmid

    #119914

    Purpose

    Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARβ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge