162,676 results

1. LT3GEP

   Plasmid

   #111172

   Purpose

   Tet-regulated miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T3G

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDH

   Plasmid

   #133129

   Purpose

   A recombinant plasmid expressing mdh (malate dehydrogenase) gene

   Depositor

   Gregg Beckham

   Article

   Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   malate dehydrogenase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   malate dehydrogenase promoter

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LeGO-dKatushka2

   Plasmid

   #85214

   Purpose

   Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   dKatushka2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   BsrGI site removed at 5' end, BsrGI site ins…

   Promoter

   SFFV

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUb PspCas13b + PspCas13b guide RNA

   Plasmid

   #176305

   Purpose

   Expresses PspCas13b and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   PspCas13b
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   E113K, N265S- please depositor comments

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Tornado-RhoBAST-MS2

   Plasmid

   #196342

   Purpose

   Overexpression of circular RhoBAST-MS2

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-RhoBAST-MS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

   Plasmid

   #55639

   Purpose

   Flp-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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