We narrowed to 5,482 results for: pcas

1. pX330-PITCh-MAP4

   Plasmid

   #227295

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of MAP4 (MAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-EZR

   Plasmid

   #227293

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of EZR (EZR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-PITCh-CEP192

   Plasmid

   #227288

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CEP192 (CEP192 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-CEP135

   Plasmid

   #227286

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PITCh-ARL13B

   Plasmid

   #227276

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of ARL13B (ARL13B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

   Plasmid

   #131683

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Inserts

   EGFP-KASH
   SpCas9 sgRNA vs mouse GRID1
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   EF1a and mU6

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPH2A.X_K5Q-GS3

   Plasmid

   #204746

   Purpose

   Expression of Cas9 and human H2AX with the K5Q mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5Q (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K5Q mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPH2A.X_K5R-GS3

   Plasmid

   #204747

   Purpose

   Expression of Cas9 and human H2AX with the K5R mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5R (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K5R mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPH2A.X_K134L-GS3

   Plasmid

   #204750

   Purpose

   Expression of Cas9 and human H2AX with the K134L mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134L (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K134L mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCPH2A.X_K134M-GS3

    Plasmid

    #204751

    Purpose

    Expression of Cas9 and human H2AX with the K134M mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134M (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134M mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPH2A.X_K134A-GS3

    Plasmid

    #204752

    Purpose

    Expression of Cas9 and human H2AX with the K134A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VIPR2-DuET

    Plasmid

    #213387

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    VIPR2 (VIPR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGMC00033 (aka pMC0224)

    Plasmid

    #210750

    Purpose

    Guide RNA targeting SERPINB3

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    SERBPINB3 guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. iCAP-BI-49-KanR

    Plasmid

    #203534

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI49 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. iCAP-BI-65-KanR

    Plasmid

    #203536

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI65 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS-dsarm(E,K450R)

    Plasmid

    #187881

    Purpose

    Gal4/UAS expression of dSarm(isoE,K450R)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm )
    

    Use

    Tags

    Expression

    Mutation

    K450R

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE_NFL linker-3xFLAG KI

    Plasmid

    #182679

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid

    #182680

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt

    Plasmid

    #175568

    Purpose

    pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    GOXL6
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    RUBY

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 NLC-FKBP-Cas9

    Plasmid

    #162726

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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