164,577 results

1. Flag-ZDHHC7

   Plasmid

   #231656

   Purpose

   Expresses human ZDHHC7 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC7 (ZDHHC7 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-miniTurbo

   Plasmid

   #124647

   Purpose

   Expresses Flag-miniTurbo fusion for proximity labeling

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. opto-RhoGEF-mCherry_pcDNA3.1

   Plasmid

   #164473

   Purpose

   Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoGEF
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD5

   Plasmid

   #73914

   Purpose

   multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   dU6-3:tRNA:gRNA:tRNA:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6:3

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-OTUB1*

   Plasmid

   #65441

   Purpose

   Expresses activated OTUB1 in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   UbcH5b (UBE2D2 Human)
   otubain-1 (OTUB1 )
   

   Use

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Active site cysteine mutated to alanine

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pOpen-DpnIR

   Plasmid

   #165529

   Purpose

   Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DpnI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Flag-Gsdmd

   Plasmid

   #80950

   Purpose

   Expresses Gsdmd in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-ATG5-K130R

   Plasmid

   #13096

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5-K130R (Atg5 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K130R

   Promoter

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK_Sox10_enh_84_Citrine

   Plasmid

   #130584

   Purpose

   enhancer reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   Sox10_enh_84
   

   Use

   Enhancer reporter

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZNRF3-SmBiT, BiBiT-Ready Vector

    Plasmid

    #237083

    Purpose

    Express ZNRF3-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ZNRF3 (ZNRF3 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid

    #207771

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-CMV-EGFP-Hygro

    Plasmid

    #184593

    Purpose

    EGFP

    Depositor

    Robert Sobol

    Article

    Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TRE-DIO-Cas9

    Plasmid

    #166601

    Purpose

    Encodes Cre-dependent Cas9 under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19 Giles integrase

    Plasmid

    #170049

    Purpose

    pUC19 with Giles integrase

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Insert

    GIles Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-M13-6His-KIF1C-GFP

    Plasmid

    #130975

    Purpose

    Expression of 6His-KIF1C-GFP in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Use

    Tags

    6His and GFP

    Expression

    Insect

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

    Plasmid

    #172985

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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