We narrowed to 9,400 results for: Coli

1. EcNGsC-S308P-H309D-pKK223

   Plasmid

   #131378

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308P H309D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcNGsC-F307A-S308A-pKK223

   Plasmid

   #131379

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307A S308A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EcNGsC-F307Y-pKK223

   Plasmid

   #131381

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307Y
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EcNGsC-F307A-pKK223

   Plasmid

   #131382

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EcNGsC-S308V-pKK223

   Plasmid

   #131393

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308V
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EcNGsC-S308A-pKK223

   Plasmid

   #131394

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29a-hNABP1NhNABP2C

   Plasmid

   #128312

   Purpose

   Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   chimeric protein hNABP1N+hNABP2C

   Promoter

   T7

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29a-hNABP1-N

   Plasmid

   #128310

   Purpose

   Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   N-terminal domain (OB fold, 1–140 aa)

   Promoter

   T7

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29a-hNABP2-C

   Plasmid

   #128309

   Purpose

   Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

   Insert

   hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal domain (122–211 aa)

   Promoter

   T7

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR357.12

    Plasmid

    #125107

    Purpose

    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A84T - see depositor comments below and Q183K-see…

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-DEST42-ARL4D-V5-His6

    Plasmid

    #67426

    Purpose

    Expression of hArl4D with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

13. hSRAP.TWIN1.a111-215

    Plasmid

    #49820

    Purpose

    expression vector for SRAP(111-215) in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 111-215 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hSRAP.TWIN1.a105-236.V105M

    Plasmid

    #49819

    Purpose

    expression vector for SRAP(105-236)V105M in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 105-236 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    V105M codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hSRAP.TWIN1.a105-215.V105M

    Plasmid

    #49818

    Purpose

    expression vector for SRAP(105-215)V105M in pTWIN1 vector

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein aa 105-215 (SRA1 Human)
    

    Use

    Tags

    intein & chitin binding domain

    Expression

    Bacterial

    Mutation

    V105M codon-optimized for E. coli expression

    Promoter

    T7

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBCSK_HIS3_II

    Plasmid

    #109059

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBCSK_HIS3_I

    Plasmid

    #109058

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBCSK_LEU2_I

    Plasmid

    #109060

    Purpose

    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes

    Depositor

    Colin Logie

    Insert

    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    LEU2

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBCSK_LEU2_II

    Plasmid

    #109061

    Purpose

    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    LEU2

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBSIIKS_HIS3_I

    Plasmid

    #109062

    Purpose

    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.

    Depositor

    Colin Logie

    Insert

    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. JD630

    Plasmid

    #160394

    Purpose

    Vitis GRF4-GIF1 in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    Vitis GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. JD689

    Plasmid

    #160396

    Purpose

    citrus GRF4-GIF1 in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    citrus GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. JD638

    Plasmid

    #160395

    Purpose

    Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    Vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. JD553

    Plasmid

    #160392

    Purpose

    Wheat GRF4-GIF1 chimera in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    wheat GRF4-GIF1 chimera
    

    Use

    Entry clon

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JD690

    Plasmid

    #160397

    Purpose

    Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::citrus GRF4-GIF1 chimera
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. JD639

    Plasmid

    #160398

    Purpose

    Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis miR396-resistant GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JD631

    Plasmid

    #160399

    Purpose

    Binary vector pGWB14 including Vitis GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::vitis GRF4-GIF1 chimera
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JD633

    Plasmid

    #160393

    Purpose

    CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Inserts

    ZmUbi::wheat GRF4-GIF1 chimera
    ZmUbi:TaCas9 + TaU6:gRNA
    

    Use

    Binary vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge