We narrowed to 24,055 results for: crispr

Showing: 5121 - 5140 of 24055 results

Results per page:

Sort results by:

pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPgRNA

Plasmid

#47108

Purpose

Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

SPgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-dCas9-BFP-KRAB

Plasmid

#46911

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP-KRAB fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, HA, and KRAB domain

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1521 (pSPIN, pSC101* backbone)

Plasmid

#160729

Purpose

Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDB_054

Plasmid

#216096

Purpose

All-in-one, knockout, delivers Cas12a & gRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001148862)

Plasmid

#80263

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PKMYT1 gRNA (BRDN0001146750)

Plasmid

#77280

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKMYT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKMYT1 (PKMYT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKMYT1 gRNA (BRDN0001146095)

Plasmid

#77278

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKMYT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKMYT1 (PKMYT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP313-pAAV-CMV-SaCas9-DIO-pA

Plasmid

#113690

Purpose

A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-dCas9-HA

Plasmid

#61355

Purpose

encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal HA tag

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

Plasmid

#113686

Purpose

An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

KRAB

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKCB gRNA (BRDN0001148507)

Plasmid

#75954

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVNP3.0-ABE8e

Plasmid

#206882

Purpose

Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

ABE8e

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

Plasmid

#140002

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1A gRNA (BRDN0001146329)

Plasmid

#77720

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1A (PRKAR1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAR1A gRNA (BRDN0001144984)

Plasmid

#77721

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAR1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAR1A (PRKAR1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge