We narrowed to 9,382 results for: APP

Showing: 5141 - 5160 of 9382 results

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-1

Plasmid

#92159

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pmCell-Free_KA2_ZIKV NS2B

Plasmid

#145364

Purpose

Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pEBB Flag GCN5

Plasmid

#74784

Purpose

express of GCN5 in mammalian cells

Depositor

Ezra Burstein

Article

Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

Insert

GCN5 (KAT2A Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-ORF4

Plasmid

#168903

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-ORF4 (orf4 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp6

Plasmid

#168895

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp6 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC cloning vector

Plasmid

#10878

Purpose

3rd generation transfer plasmid

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC control

Plasmid

#10879

Purpose

Negative control shRNA vector containing non-hairpin insert.

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Insert

non-hairpin 18bp

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-bTrCP

Plasmid

#20718

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

beta-transducin repeat containing (BTRC) (Btrc Mouse)

Tags

Myc3

Expression

Mammalian

Mutation

*

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

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MAC_N_GSK3B

Plasmid

#111687

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

GSK3B (GSK3B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET-pA-Hia5

Plasmid

#174372

Purpose

SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein A - hia5

Tags

6xHis and Protein A

Expression

Bacterial

Available Since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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