We narrowed to 15,731 results for: LEA

1. pCAGGS-PB1-MontiRed-LOV2dark

   Plasmid

   #178874

   Purpose

   Mammalian expression of PB1-MontiRed-LOV2dark

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-MontiRed-LOV2dark
   

   Expression

   Mammalian

   Mutation

   LOV2: C450A, L514K, G528A, L531E, N538E

   Promoter

   CAG

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52537

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30b GluBP600n

   Plasmid

   #119835

   Purpose

   Production of recombinant GluBP600n high affinity protein in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   GluBP600n
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. M48 GST-TEV-Chibby-N

   Plasmid

   #17196

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   Chibby (CBY1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-63

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52538

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProvector_AAV2_586_PLGLAR

   Plasmid

   #129024

   Purpose

   Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

   Depositor

   Junghae Suh

   Article

   Tong et al J Control Release. 2019 Jun 25. pii: S01

   Insert

   Rep2/Cap2 with PLGLAR lock at 586
   

   Use

   AAV

   Mutation

   inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS1 DICER1 short UTR

   Plasmid

   #21648

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET41a iGlul

   Plasmid

   #119832

   Purpose

   Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGlul
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T92A

   Promoter

   T7

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-hB50

   Plasmid

   #18967

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican N-terminus (BCAN Human)
   

   Expression

   Mammalian

   Mutation

   Human brevican N-terminus aa 1-400

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC6

    Plasmid

    #118864

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, EGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA6/ERGIC2-WT

    Plasmid

    #51479

    Purpose

    Expresses ERGIC2-WT in mammalian cells

    Depositor

    Simon Kwok

    Article

    Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

    Insert

    ERGIC2-WT (ERGIC2 Human)
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

13. M49 GST-TEV-Chibby-C

    Plasmid

    #17197

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    Chibby (CBY1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 64-126

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pIS1 Cyclin D2 long-mut-miR15/16

    Plasmid

    #21647

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 long-mut-miR15/16 (CCND2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR15/16 microRNA binding sites

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ets2DBM

    Plasmid

    #28129

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    Ets2 (ETS2 Human)
    

    Use

    Mouse Targeting and Retroviral

    Expression

    Mammalian

    Mutation

    The putative destruction box (RGTL) residues 20–2…

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pOCC81

    Plasmid

    #118900

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease; GST tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD4_LD 0_CS G_tTA-BFP

    Plasmid

    #58869

    Purpose

    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMU103

    Plasmid

    #73934

    Purpose

    This is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.

    Depositor

    Zhanyuan Zhang

    Article

    Flores et al Transgenic Res. 2008 Oct;17(5):839-50. d

    Insert

    bar and GUS cassettes
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. M47 MBP-TEV-Chibby

    Plasmid

    #17195

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    Chibby (CBY1 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only