We narrowed to 5,878 results for: pCas

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pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-MLH1dn-2A-BSD

Plasmid

#234072

Purpose

For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax-2A-MLH1dn

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEBB Flag GCN5

Plasmid

#74784

Purpose

express of GCN5 in mammalian cells

Depositor

Ezra Burstein

Article

Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

Insert

GCN5 (KAT2A Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Cloning template vector

Plasmid

#131471

Purpose

Cloning template form ORANGE method based knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pUBQ:Citrine-TurboID-3xMyc

Plasmid

#199244

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID-3xMyc

Tags

TurboID-3xMyc

Expression

Plant

Promoter

Arabidopsis UBQ10 promoter with intron

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Article

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-GFP

Plasmid

#159654

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MSP1830

Plasmid

#70708

Purpose

Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Insert

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

CAG

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBG-ttLbUV2

Plasmid

#231386

Purpose

LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

Depositor

Qi-Jun Chen

Article

Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

Insert

ttLbCas12a Ultra V2

Use

CRISPR

Expression

Plant

Mutation

D156R and E795L

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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PEmaxNG-_2star_IVT

Plasmid

#227678

Purpose

plasmid for SpCas9-NG-PEmax** mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

NG-PEmax_2_star

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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