We narrowed to 5,822 results for: pCas

1. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-NRCH-PE2max-BSD

   Plasmid

   #191103

   Purpose

   Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PEmax
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9-NRCH

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FZ-NbPDS

   Plasmid

   #184268

   Purpose

   pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

   Depositor

   Feng Qu

   Article

   Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

   Insert

   CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
   

   Expression

   Plant

   Mutation

   The protease processing site between MP and L-CP …

   Promoter

   CaMV 35S with duplicated enhancer

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459-TDP-43 sgRNA1

   Plasmid

   #133762

   Purpose

   Expresses Cas9 and human TDP-43 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, EF-1a

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330 _CRISPIE donor B8

   Plasmid

   #172849

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FL MALT1 WT in pET29b-Strep

    Plasmid

    #48968

    Purpose

    Expresses Full length wild type MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Tags

    His and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pRvCAHS3-CAHS3-mEGFP

    Plasmid

    #205006

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    RvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-TSF-TEVcs-ULK1K46I

    Plasmid

    #171417

    Purpose

    For the purifcation of human ULK1 kinase dead complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    kinase dead

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRvSAHS1-SAHS1-mEGFP

    Plasmid

    #205007

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    RvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRvTUB-mEGFP-NLS

    Plasmid

    #205011

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Target-AIDmax

    Plasmid

    #139107

    Purpose

    Mammalian Target-AIDmax expression vector (pSI-626)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. px459-TDP 43 sgRNA2

    Plasmid

    #133763

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330 _CRISPIE donor B7

    Plasmid

    #172848

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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