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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S285C

Plasmid

#98666

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S285C D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S329C

Plasmid

#98668

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

D290V S329C

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R201K_R216K_R254K

Plasmid

#104467

Purpose

express MBP hnRNPA2 LC with 4 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R201K R216K R254K

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + Z-AAT target

Plasmid

#86007

Purpose

lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
Z-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + TTR target

Plasmid

#86009

Purpose

lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
TTR target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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E2AK2_HUMAN_D0

Plasmid

#79712

Purpose

This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

E2AK2 (EIF2AK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FASTK gRNA (BRDN0001149199)

Plasmid

#77878

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G596C

Plasmid

#131712

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G596C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469S

Plasmid

#131716

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469S

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600R

Plasmid

#131721

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-hPE(+)-Luc

Plasmid

#172003

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-hPE(-)-Luc

Plasmid

#172004

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-mPE(-)-Luc

Plasmid

#172006

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Reverse Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-mPE(+)-Luc

Plasmid

#172005

Purpose

Luciferase Reporter for Mouse PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Mouse PE sequence (mPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHis-3xFLAG-CMV14-CYB5R3 K214R

Plasmid

#204552

Purpose

Expresses 3xFLAG-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Mammalian

Mutation

K214R

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-puro /BRAF-V600E

Plasmid

#131724

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-pAzF[TAG]

Plasmid

#164579

Purpose

Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

araBAD, glnS, and proK

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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EIF2AK2 gRNA (BRDN0001148577)

Plasmid

#75638

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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