We narrowed to 18,727 results for: MUT
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Plasmid#229423PurposeLentiviral or overpexression of cDNADepositorInsertMARK2-GK-CR (MARK2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGatekeeper mutation M129G; silent mutation CRISPR…PromoterEFSAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron3_sg3_pX458
Plasmid#127338PurposesgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3DepositorInsertMouse Cdk13 Intron 3 sgRNA
UseCRISPR and Mouse TargetingPromoterU6 PromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron4_sg4_pX330
Plasmid#127341PurposesgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4DepositorInsertMouse Cdk13 Intron 4 Targeting sgRNA
UseCRISPR and Mouse TargetingPromoterU6 PromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps-Puro-FLAG-HA-Regnase-3 D252N
Plasmid#222664PurposeLentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterSFFVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.CBX8-KR21A
Plasmid#224709PurposeExpresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcs2-cDELE1(142-515)-sumo
Plasmid#226107PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assaysDepositorInsertDELE1 (DELE1 Human)
TagsSumoExpressionMammalianMutationencodes only residues 142-515 of DELE1 (represent…Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
TagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
gamma-tubulin (1-335)
Plasmid#226519PurposeExpressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domainDepositorInsertTUBG1 N-terminal amino acids 1-335 (TUBG1 Human)
UsePcdna3.1ExpressionMammalianMutationDeleted amino acids 334-451 and introduced the fo…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Regnase-3 D252N-T2A-copGFP
Plasmid#222661PurposeLentiviral vector to express mouse Regnase-3 D252NDepositorInsertZc3h12c (Zc3h12c Mouse)
UseLentiviralExpressionMammalianMutationmutated aspartic acid 252 to asparagine to abroga…PromoterEF1αAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N
Plasmid#222650PurposeFor in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-termDepositorInsertZc3h12a (Zc3h12a Mouse)
UseIn vitro transcriptionTagsFLAG and HAMutationmutated aspartic acid 141 to asparagine to abroga…PromoterT7Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK189
Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt1
Plasmid#220239PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationR27A, R31A, F165APromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG
Plasmid#214706PurposeMammalian expression of human CALR ins5 YD/FL-FLAGDepositorAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) T59/T61 A
Plasmid#195032PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
ExpressionMammalianMutationT59/T61 AAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) C18/C23 S
Plasmid#195033PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
ExpressionMammalianMutationC18/C23 SAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S172A_M173A_V195A RAD23A
Plasmid#201576PurposeExpresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
TagsFLAGExpressionMammalianMutationContains mutations S172A, M173A and V195AAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q)
Plasmid#200979PurposeMammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
TagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q mutation fo…PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-hCIT
Plasmid#203166PurposeYeast expression vector for human DHDDSDepositorAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2C-ΔC-S230A
Plasmid#200561PurposeSecreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
UseAAVTagsFlagPromoterTBG promoterAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T
Plasmid#147590PurposeInsect Expression of DmThor-CG32016-EDepositorInsertDmThor-CG32016-E (Thor Fly)
ExpressionInsectMutationDmThor two silent mutations compared to the seque…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1710-2505_J
Plasmid#146701PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
ExpressionInsectMutationone non silent mutation (A1747T) and one silent m…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-delLN-DmPABPC1-V5His6_H
Plasmid#146477PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
Plasmid#146862PurposeInsect Expression of DmPABPC1_del183-258-siRNAresDepositorInsertDmPABPC1_del183-258-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L
Plasmid#146863PurposeInsect Expression of DmPABPC1_del288-359-siRNAresDepositorInsertDmPABPC1_del288-359-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and six silent muta…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L
Plasmid#146864PurposeInsect Expression of DmPABPC1_del364-550-siRNAresDepositorInsertDmPABPC1_del364-550-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and five silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-ND-Cdt1-mCitrine
Plasmid#193771PurposeHuman Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCitrine (CDT1 Human)
TagsmCitrineExpressionMammalianMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only