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pFastBac Dual α1B/β3 Tubulin

Plasmid

#170552

Purpose

Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB3 (TUBB3 Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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BNLS

Plasmid

#50836

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP B

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-F656W

Plasmid

#72546

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F656W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-I654Q

Plasmid

#72545

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I654Q

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-L630W

Plasmid

#72543

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L630W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Tubulin-C-18

Plasmid

#231771

Purpose

Visualization of tubulin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-M2-GPHN

Plasmid

#31665

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Gephyrin (GPHN Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry

Plasmid

#35510

Purpose

AAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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GANLS

Plasmid

#50852

Purpose

Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-hARdeltaNH2-6

Plasmid

#89104

Purpose

mammalian expression of human androgen receptor amino acids 507-919

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR DNA binding domain+ligand binding domain (AR Human)

Expression

Mammalian

Mutation

NH2 terminal deletion, contains amino acids 507-…

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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RANLS

Plasmid

#50843

Purpose

Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Article

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pCIneo-lambdaN-HA-HsEDC4

Plasmid

#66597

Purpose

Expresses lambdaN-HA-tagged EDC4 in mammalian cells

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC4 (EDC4 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD24-T2A-GFP

Plasmid

#205448

Purpose

lentiviral plasmid for expression of mouse CD24

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD24 (Cd24a Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-Qa1 SCT-T2A-GFP

Plasmid

#205453

Purpose

lentiviral plasmid for expression of mouse Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Qa1 SCT (H2-T23 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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