We narrowed to 9,265 results for: yeast expression

1. miniTurbo-V5_pRS415

   Plasmid

   #107168

   Purpose

   expresses V5-tagged miniTurbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIW601-KmCRISPR

   Plasmid

   #98907

   Purpose

   K. marxianus CRISPR Plasmid for sgRNA cloning

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCTCON2 Aga2P-HRP

   Plasmid

   #73152

   Purpose

   Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Aga2P-HRP
   

   Use

   Tags

   HA tag and myc tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPEY-GAL-Nat

   Plasmid

   #232097

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSc-humanADAR2

   Plasmid

   #171106

   Purpose

   Expresses human ADAR2 in yeast.

   Depositor

   Brenda Bass

   Article

   Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

   Insert

   human ADAR2 (ADARB1 Human)
   

   Use

   Tags

   His12_TEV

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPEY-GAL-Kan

   Plasmid

   #232099

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPEY-GAL-Hyg

   Plasmid

   #232098

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPEY-Z3-Nat

    Plasmid

    #232100

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsNXF-1_K

    Plasmid

    #146740

    Purpose

    Mammalian Expression of HsNxf-1

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsNxf-1 (NXF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S144G relative to NM_006362.4

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS406 ATG9 stopGFP

    Plasmid

    #114669

    Purpose

    Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

    Insert

    ATG9
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Changed the three linker base pairs after the las…

    Promoter

    ATG9

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS406-PHO5-GFP-OSBP PH domain

    Plasmid

    #58840

    Purpose

    Expresses GFP-Tagged OSBP PH domain in yeast

    Depositor

    Sean Munro, Tim Levine

    Article

    Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

    Insert

    Oxysterol-binding Protein PH domain (OSBP Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNS1

    Plasmid

    #131769

    Purpose

    PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    FUSN_Fusion Red_Cry2WT
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PADH1

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only