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We narrowed to 1,231 results for: vent

Showing: 1001 - 1040 of 1231 results

  13. Myc-Bnip3-T181A

    Plasmid
    #197556
    Purpose
    Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)
    Depositor
    Joseph Gordon
    Article
    Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.
    Insert
    BCL2 Interacting Protein 3 (Bnip3 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Threonine 181 changed to alanine
    Promoter
    CMV
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197556-plasmid-map-sequence-id-386257

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  14. pEMS1132

    Plasmid
    #29053
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29053-plasmid-map-sequence-id-13468

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  15. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  19. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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  20. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  23. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid
    #187936
    Purpose
    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327
    Depositor
    Sascha Martens
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Use
    Tags
    GST-mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187936-plasmid-map-sequence-id-374446

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  24. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

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  25. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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  26. pET41-FeoB

    Plasmid
    #216753
    Purpose
    Overexpression of FeoB for biological characterisation
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Wild type
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    216753-plasmid-map-sequence-id-447881

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  27. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

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  28. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

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  29. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

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  30. pBS3ndd

    Plasmid
    #97354
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Tags
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97354-plasmid-map-sequence-id-191848

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  31. pBS2ndd

    Plasmid
    #97353
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Tags
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97353-plasmid-map-sequence-id-190845

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  32. PKA1317

    Plasmid
    #239371
    Purpose
    S.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3)
    Depositor
    Kathryn Ayscough
    Insert
    SLA1 (5-131)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    W108A
    Promoter
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239371-plasmid-map-sequence-id-478756

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  33. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  34. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

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  35. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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Showing: 1001 - 1040 of 1231 results