We narrowed to 881 results for: nanobody

1. pcDNA5-FRT/TO-APT1-LaG16-3W

   Plasmid

   #234424

   Purpose

   Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. WIW_Scaffold2

   Plasmid

   #205574

   Purpose

   Nanobody that inhibits seeding of tau fibrils

   Depositor

   David Eisenberg

   Article

   Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   WIW_Scaffold2
   

   Tags

   6X His

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-N1 GBP (GBP-mCherry)

   Plasmid

   #162879

   Purpose

   Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   GFP Binding Protein
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-HGP-v3-Nb127D01-hIgG

   Plasmid

   #171564

   Purpose

   Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
   

   Tags

   BiP signal peptide and hIgG1-hinge-N297A-TEV site…

   Expression

   Insect

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINVHH_H6_His

   Plasmid

   #201719

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_H6 (S )
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE-tdTomato

   Plasmid

   #50798

   Purpose

   Expression of tdTomato under the control of the TRE-promoter

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   TREtight driven tdTomato fluorescent protein
   

   Use

   Mutli-species

   Promoter

   TREtight

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBMNb-cALFA

   Plasmid

   #159991

   Purpose

   For the protein expression with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   CMV promoter, WPRE

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-miRFP670nano3

    Plasmid

    #184664

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. nls-mCherry

    Plasmid

    #108881

    Purpose

    Expression of nuclear localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

    Plasmid

    #162230

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINVHH_his_flag

    Plasmid

    #210404

    Purpose

    For expression of VHH with C-terminal his and Flag tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Tags

    his6-flag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Tags

    Avi and His

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-CaaX(Hras)

    Plasmid

    #108886

    Purpose

    Expression of membrane localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    mCherry-CaaX(Hras)
    

    Use

    Can be used for mammalian expression or in vitro …

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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