We narrowed to 2,540 results for: organization Addgene

1. pJJR83

   Plasmid

   #75028

   Purpose

   mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   mCherry^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized mCherry

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV5B+longBRD4

   Plasmid

   #157792

   Purpose

   Expression of long isoform of BRD4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   long BRD4 (BRD4 Human)
   

   Tags

   His6

   Expression

   Insect

   Promoter

   polyhedron

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM-DCA-muGFP

   Plasmid

   #166727

   Purpose

   AAV, encodes monomeric ultrastable GFP (muGFP)

   Depositor

   Daniel Scott

   Article

   Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

   Insert

   muGFP
   

   Use

   AAV

   Promoter

   Chicken Beta Actin

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Lifeact-mRuby

   Plasmid

   #194339

   Purpose

   Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Lifeact
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-DCX-2A-mCherry

   Plasmid

   #118290

   Purpose

   Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   doublecortin (DCX Human)
   

   Use

   AAV

   Tags

   2A peptide and mCherry

   Expression

   Mammalian

   Promoter

   CMV with beta global intron

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

   Plasmid

   #179586

   Purpose

   ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

   Depositor

   Ali Hemmati Brivanlou

   Article

   De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

   Insert

   NLS-NG-T2A-hSHH
   

   Use

   Piggybac

   Tags

   NeonGreen

   Promoter

   TRE

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBC_HA_PP7-mVenus

   Plasmid

   #111268

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mVenus
   

   Use

   Synthetic Biology

   Tags

   HA, SV40 NLS, and mVenus

   Expression

   Mammalian

   Mutation

   PP7: C68S, S69G [delta]70-75 (non-aggregating)

   Promoter

   hUBC

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-ML1N*2

    Plasmid

    #67678

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-mRuby-Rab8a

    Plasmid

    #194337

    Purpose

    Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Rab8 (RAB8A Canis lupus)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. PA-mCherry-miniSOG-Paxillin-22

    Plasmid

    #57796

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Paxillin
    

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PA-mCherry-miniSOG-Lysosomes-20

    Plasmid

    #57793

    Purpose

    Localization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LAMP1
    

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    D50E in LAMP1

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. K401-Micro

    Plasmid

    #122485

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    His tag MBP Kinesin 1-401 SspB micro
    

    Tags

    H6-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mKate-P2A-APEX2-NS

    Plasmid

    #67670

    Purpose

    Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-NS
    

    Use

    Gateway multisite middle entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2mFoxd3

    Plasmid

    #37267

    Depositor

    Patricia Labosky

    Article

    Steiner et al Development. 2006 Dec;133(24):4827-38. E

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Overexpression

    Expression

    Mammalian

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only