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We narrowed to 2,542 results for: pcas9

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Showing: 501 - 520 of 2542 results

  3. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  9. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid
    #106964
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    106964-plasmid-map-sequence-id-207987

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  11. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Article
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  15. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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  17. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid
    #210391
    Purpose
    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)
    Depositor
    Benjamin Kleinstiver
    Article
    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:
    Insert
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    CamKII promoter for Cre, human U6 promoter for Sp…
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    210391-plasmid-map-sequence-id-415458

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  19. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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Showing: 501 - 520 of 2542 results