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C32V

Plasmid

#26396

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-32-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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Cas9 MDC123

Plasmid

#59184

Purpose

2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

2X35S

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. CD274

Plasmid

#107003

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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aMHC-Puro Rex-Blast

Plasmid

#21231

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-CHC17KDP

Plasmid

#59799

Purpose

Expresses knockdown-proof human CHC17 tagged with GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

CHC17 (CLTC Human)

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 61-65 to confer s…

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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2.2

Plasmid

#34885

Depositor

Irvin Chen

Article

Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

Insert

Sindbis virus envelope protein

Use

Lentiviral

Expression

Mammalian

Mutation

SGN UTR in E1 domain

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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C17V

Plasmid

#26395

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-17-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

Plasmid

#162279

Purpose

Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN2A Variant 1, stabilized (SCN2A Human)

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp4551.

Promoter

CMV

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9P35S-B

Plasmid

#66190

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Flag-Timeless

Plasmid

#22887

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Mammalian

Mutation

deleted normal start codon, replaced with start c…

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla

Plasmid

#60510

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-Turbo-YFP-NLS

Plasmid

#127368

Purpose

Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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